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  A DNA-unwinding enzyme induced in bacteriophage-T4-infected Escherichia coli cells

Krell, H., Dürwald, H., & Hoffmann-Berling, H. (1979). A DNA-unwinding enzyme induced in bacteriophage-T4-infected Escherichia coli cells. European Journal of Biochemistry, 93(2), 387-395. doi:10.1111/j.1432-1033.1979.tb12835.x.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-55A7-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-55A8-F
Genre: Journal Article

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EurJBiochem_93_1979_387.pdf (Any fulltext), 750KB
 
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 Creators:
Krell, Herbert1, Author              
Dürwald, Hildegard1, Author              
Hoffmann-Berling, Hartmut2, Author              
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1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              
2Department of Molecular Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497702              

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 Abstract: A single-stranded DNA-dependent ATP gamma-phosphohydrolase of Mr 56000 induced after infection of Escherichia coli cells with bacteriophage T4, probably the ATPase dependent on gene dda of the phage, was isolated. Studies on the enzyme show that in the presence of ATP and M2+ ions it is capable of dissociating partially double-stranded fd bacteriophage DNA into the single strands and that some 3000 enzyme copies are required to unwind the 6400-nucleotides-long DNA. Unwinding is inhibited by reducing the length of the single-stranded portion of DNA to two nucleotides. In addition it can be inhibited by sulfhydryl reagents which block the ATPase or by trapping free enzyme molecules in the assay system. The results suggest that unwinding is initiated near the single-stranded portion of the DNA and is driven by the ATPase. It further appears that the enzyme unwinds by adsorbing to the DNA. Affinity of the enzyme for double-standed DNA is not detectable by DNA binding assay.

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Language(s): eng - English
 Dates: 1978-09-082008-06-281979-01
 Publication Status: Published in print
 Pages: 9
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 Table of Contents: -
 Rev. Method: Peer
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Title: European Journal of Biochemistry
Source Genre: Journal
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Publ. Info: Berlin : Published by Springer-Verlag on behalf of the Federation of European Biochemical Societies
Pages: - Volume / Issue: 93 (2) Sequence Number: - Start / End Page: 387 - 395 Identifier: ISSN: 0014-2956
CoNE: https://pure.mpg.de/cone/journals/resource/111097776606040