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  Combined use of 16S ribosomal DNA and 16S rRNA to study the bacterial community of polychlorinated biphenyl-polluted soil

Nogales, B., Moore, E. R. B., Llobet-Brossa, E., Rosselló-Mora, R., Amann, R. I., & Timmis, K. N. (2001). Combined use of 16S ribosomal DNA and 16S rRNA to study the bacterial community of polychlorinated biphenyl-polluted soil. Applied and Environmental Microbiology, 67(4), 1874-1884.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-55F4-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-55F5-8
Genre: Journal Article

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Nogales, Balbina , Author
Moore, Edward R. B. , Author
Llobet-Brossa, Enrique1, Author              
Rosselló-Mora, Ramon1, Author              
Amann, Rudolf I.1, Author              
Timmis, Kenneth N. , Author
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1Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481696              

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 Abstract: The bacterial diversity assessed from clone libraries prepared from rRNA (two libraries) and ribosomal DNA (rDNA) (one library) from polychlorinated biphenyl (PCB)-polluted soil has been analyzed. A good correspondence of the community composition found in the two types of library was observed. Nearly 29% of the cloned sequences in the rDNA library were identical to sequences in the rRNA libraries. More than 60% of the total cloned sequence types analyzed were grouped in phylogenetic groups (a clone group with sequence similarity higher than 97% [98% for Burkholderia andPseudomonas-type clones]) represented in both types of libraries. Some of those phylogenetic groups, mostly represented by a single (or pair) of cloned sequence type(s), were observed in only one of the types of library. An important difference between the libraries was the lack of clones representative of the Actinobacteriain the rDNA library. The PCB-polluted soil exhibited a high bacterial diversity which included representatives of two novel lineages. The apparent abundance of bacteria affiliated to the beta-subclass of theProteobacteria, and to the genus Burkholderiain particular, was confirmed by fluorescence in situ hybridization analysis. The possible influence on apparent diversity of low template concentrations was assessed by dilution of the RNA template prior to amplification by reverse transcription-PCR. Although differences in the composition of the two rRNA libraries obtained from high and low RNA concentrations were observed, the main components of the bacterial community were represented in both libraries, and therefore their detection was not compromised by the lower concentrations of template used in this study.

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Language(s): eng - English
 Dates: 2001-04
 Publication Status: Published in print
 Pages: 11
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Title: Applied and Environmental Microbiology
  Other : Appl. Environ. Microbiol.
Source Genre: Journal
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Publ. Info: American Society for Microbiology (ASM)
Pages: 11 Volume / Issue: 67 (4) Sequence Number: - Start / End Page: 1874 - 1884 Identifier: ISSN: 0099-2240
CoNE: https://pure.mpg.de/cone/journals/resource/954927519600