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  Leishmania tarentolae: taxonomic classification and its application as a promising biotechnological expression host

Klatt, S., Simpson, L., Maslov, D. A., & Konthur, Z. (2019). Leishmania tarentolae: taxonomic classification and its application as a promising biotechnological expression host. PLoS Neglected Tropical Diseases, 13(7): e0007424. doi:10.1371/journal.pntd.0007424.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-6280-C Version Permalink: http://hdl.handle.net/21.11116/0000-0004-6281-B
Genre: Journal Article

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Klatt, Stephan1, Author              
Simpson, Larry, Author
Maslov, Dmitri A., Author
Konthur, Zoltán1, Author              
Affiliations:
1Zoltán Konthur, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863499              

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 Abstract: In this review, we summarize the current knowledge concerning the eukaryotic protozoan parasite Leishmania tarentolae, with a main focus on its potential for biotechnological applications. We will also discuss the genus, subgenus, and species-level classification of this parasite, its life cycle and geographical distribution, and similarities and differences to human-pathogenic species, as these aspects are relevant for the evaluation of biosafety aspects of L. tarentolae as host for recombinant DNA/protein applications. Studies indicate that strain LEM-125 but not strain TARII/UC of L. tarentolae might also be capable of infecting mammals, at least transiently. This could raise the question of whether the current biosafety level of this strain should be reevaluated. In addition, we will summarize the current state of biotechnological research involving L. tarentolae and explain why this eukaryotic parasite is an advantageous and promising human recombinant protein expression host. This summary includes overall biotechnological applications, insights into its protein expression machinery (especially on glycoprotein and antibody fragment expression), available expression vectors, cell culture conditions, and its potential as an immunotherapy agent for human leishmaniasis treatment. Furthermore, we will highlight useful online tools and, finally, discuss possible future applications such as the humanization of the glycosylation profile of L. tarentolae or the expression of mammalian recombinant proteins in amastigotelike cells of this species or in amastigotes of avirulent human-pathogenic Leishmania species.

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 Dates: 2019-07-252019
 Publication Status: Published in print
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 Identifiers: DOI: 10.1371/journal.pntd.0007424
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Title: PLoS Neglected Tropical Diseases
Source Genre: Journal
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Publ. Info: Cambridge, UK : Public Library of Science
Pages: - Volume / Issue: 13 (7) Sequence Number: e0007424 Start / End Page: - Identifier: ISSN: 1935-2735