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  13. Giant vesicles: a biomimetic tool for assessing membrane material properties and interactions

Steinkühler, J., & Dimova, R. (2019). 13. Giant vesicles: a biomimetic tool for assessing membrane material properties and interactions. In M.-P. Nieh, & F. A. Heberle (Eds.), Characterization of biological membranes: structure and dynamics (pp. 415-440). Berlin/Munich/Boston: Walter de Gruyter GmbH.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-6953-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-6954-8
Genre: Book Chapter

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 Creators:
Steinkühler, Jan1, Author              
Dimova, Rumiana1, Author              
Affiliations:
1Rumiana Dimova, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863328              

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 Abstract: Giant unilamellar vesicles (GUVs) have sizes in the range of 10-100 μm, which defines their unique property: they are visible under a light microscope. GUVs provide a handy biomimetic tool for directly displaying the response of the membrane on the cellsize scale. They represent model biomembrane systems for systematic measurements of mechanical and rheological properties of lipid bilayers as a function of membrane composition and phase state, surrounding media, and temperature. Here, we first summarize methods for preparing GUVs and their observation. Then, we introduce different experimental techniques which can yield precise values of membrane material characteristics such as mechanical properties (bending rigidity, stretching elasticity, lysis tension, and spontaneous curvature) and rheology (fluidity and viscosity of the membrane). Design, setup, practical tips, and evaluation of such experiments are discussed. An example on vesicle immobilization facilitating such measurements is also introduced.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1515/9783110544657-013
 Degree: -

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Title: Characterization of biological membranes : structure and dynamics
Source Genre: Book
 Creator(s):
Nieh, Mu-Ping, Editor
Heberle, Frederick A., Editor
Katsaras, John, Author
Affiliations:
-
Publ. Info: Berlin/Munich/Boston : Walter de Gruyter GmbH
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 415 - 440 Identifier: ISBN: 9783110544657
DOI: 10.1515/9783110544657