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  FRAP Analysis of Extracellular Diffusion in Zebrafish Embryos

Müller, P., & Soh, G. (2018). FRAP Analysis of Extracellular Diffusion in Zebrafish Embryos. In Methods in Molecular Biology. US: Springer.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0004-652E-8 Version Permalink: https://hdl.handle.net/21.11116/0000-000A-591C-4
Genre: Book Chapter

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 Creators:
Müller, P1, Author           
Soh, GH1, Author           
Affiliations:
1Müller Group, Friedrich Miescher Laboratory, Max Planck Society, Max-Planck-Ring 9, 72076 Tübingen, DE, ou_3008690              

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 Abstract: Morphogens are signaling molecules that provide positional information to cells during development. They must move through embryonic tissues in order to coordinate patterning. The rate of a morphogen's movement through a tissue-its effective diffusivity-affects the morphogen's distribution and therefore influences patterning. Fluorescence recovery after photobleaching (FRAP) is a powerful method to measure the effective diffusion of molecules through cells and tissues, and has been successfully employed to examine morphogen mobility and gain important insights into embryogenesis. Here, we provide detailed protocols for FRAP assays in vitro and in living zebrafish embryos, and we explain how to analyze FRAP data using the open-source software PyFRAP to determine effective diffusion coefficients.

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Language(s): eng - English
 Dates: 2018
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-1-4939-8772-6_6
PMID: 30324594
 Degree: -

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Title: Methods in Molecular Biology
  Other : Methods Mol. Biol.
Source Genre: Series
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Publ. Info: US : Springer
Pages: - Volume / Issue: 1863 Sequence Number: - Start / End Page: - Identifier: ISSN: 1064-3745
CoNE: https://pure.mpg.de/cone/journals/resource/954927725544

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Title: Morphogen Gradients: Methods and Protocols
Source Genre: Book
 Creator(s):
Dubrulle, J, Editor
Affiliations:
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Publ. Info: New York, NY, USA : Springer
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 107 - 124 Identifier: ISBN: 978-1-4939-8772-6