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  The pause-initiation limit restricts transcription activation in human cells.

Gressel, S., Schwalb, B., & Cramer, P. (2019). The pause-initiation limit restricts transcription activation in human cells. Nature Communicationsvolume, 10(1): 3603. doi:10.1038/s41467-019-11536-8.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-6D4F-B Version Permalink: http://hdl.handle.net/21.11116/0000-0004-6D96-9
Genre: Journal Article

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 Creators:
Gressel, S.1, Author              
Schwalb, B.1, Author              
Cramer, P.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: Eukaryotic gene transcription is often controlled at the level of RNA polymerase II (Pol II) pausing in the promoter-proximal region. Pausing Pol II limits the frequency of transcription initiation ('pause-initiation limit'), predicting that the pause duration must be decreased for transcriptional activation. To test this prediction, we conduct a genome-wide kinetic analysis of the heat shock response in human cells. We show that the pause-initiation limit restricts transcriptional activation at most genes. Gene activation generally requires the activity of the P-TEFb kinase CDK9, which decreases the duration of Pol II pausing and thereby enables an increase in the productive initiation frequency. The transcription of enhancer elements is generally not pause limited and can be activated without CDK9 activity. Our results define the kinetics of Pol II transcriptional regulation in human cells at all gene classes during a natural transcription response.

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Language(s): eng - English
 Dates: 2019-08-09
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41467-019-11536-8
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Title: Nature Communicationsvolume
Source Genre: Journal
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Pages: 12 Volume / Issue: 10 (1) Sequence Number: 3603 Start / End Page: - Identifier: -