English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Morphine and Endomorphins Differentially Regulate μ-Opioid Receptor mRNA in SHSY-5Y Human Neuroblastoma Cells

Yu, X., Mao, X., Blake, A., Li, W., & Chang, S. (2003). Morphine and Endomorphins Differentially Regulate μ-Opioid Receptor mRNA in SHSY-5Y Human Neuroblastoma Cells. Journal of Pharmacology and Experimental Therapeutics, 306(2), 447-454. doi:10.1124/jpet.103.048694.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0004-6EF4-E Version Permalink: http://hdl.handle.net/21.11116/0000-0004-730D-D
Genre: Journal Article

Files

show Files

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Yu, X1, Author              
Mao, X, Author
Blake, AD, Author
Li, WX, Author
Chang, SL, Author
Affiliations:
1External Organizations, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: A sensitive quantitative-competitive reverse transcriptase-polymerase chain reaction method was developed to measure μ-opioid receptor (MOR) mRNA expression in SHSY-5Y neuroblastoma cells. Differentiation of SHSY-5Y cells with either retinoic acid (RA) or 12-o-tetradecanoyl-phorbol-13-acetate (TPA) significantly increased MOR mRNA levels. Morphine treatment (10 μM) for 24 h decreased MOR mRNA levels in control, as well as RA- and TPA-differentiated cells. In contrast, chronic exposure to the opioid peptides endomorphin-1 or endomorphin-2 significantly increased MOR mRNA levels in undifferentiated and RA-differentiated cells. An opioid antagonist, naloxone, reversed the morphine and endomorphin-1 and -2 effects on MOR mRNA levels in undifferentiated SHSY-5Y cells, but naloxone had differential reversing effects on the agonists' regulation of MOR mRNA in RA- or TPA-differentiated cells. To investigate whether the changes in MOR mRNA expression paralleled changes in MOR receptor function, intracellular cAMP accumulation in SHSY-5Y cells was measured. After chronic treatment with morphine, forskolin-induced cAMP levels in SHSY-5Y cells were significantly higher than those of untreated control cells. In contrast, forskolin-induced cAMP accumulation levels were lower in cells treated with endomorphin-1 or -2 than in untreated control cells. Together, our studies indicate that the opioid alkaloid morphine and the opioid peptides endomorphin-1 and -2 differentially regulate MOR mRNA expression and MOR function in SHSY-5Y cells.

Details

show
hide
Language(s):
 Dates: 2003-08
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1124/jpet.103.048694
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Pharmacology and Experimental Therapeutics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Bethesda, Md. : American Society for Pharmacology and Experimental Therapeutics
Pages: - Volume / Issue: 306 (2) Sequence Number: - Start / End Page: 447 - 454 Identifier: ISSN: 0022-3565
CoNE: https://pure.mpg.de/cone/journals/resource/954925432383