English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Imaging Reporter Strategy to Monitor Gene Activation of Microglia Polarisation States under Stimulation

Collmann, F. M., Pijnenburg, R., Schneider, G., Schafer, C., Folz-Donahue, K., Kukat, C., et al. (2018). Imaging Reporter Strategy to Monitor Gene Activation of Microglia Polarisation States under Stimulation. J Neuroimmune Pharmacol, 13(3), 371-382. doi:10.1007/s11481-018-9789-2.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0004-7201-A Version Permalink: http://hdl.handle.net/21.11116/0000-0004-766B-0
Genre: Journal Article

Files

show Files

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Collmann, F. M.1, Author
Pijnenburg, R.1, Author
Schneider, G.1, Author
Schafer, C.1, Author
Folz-Donahue, K.1, Author
Kukat, C.1, Author
Hoehn, M.1, Author
Affiliations:
1Max Planck Institute for Biology of Ageing, Max Planck Society, Joseph-Stelzmann-Str. 9b, D-50931 Cologne, DE, ou_1942284              

Content

show
hide
Free keywords: Bioluminescence imaging Fluorescence imaging Microglia Microglia polarisation Reporter imaging
 Abstract: Microglial cells as innate immune key players have a critical and unique role in neurodegenerative disorders. They strongly interact with their microenvironment in a complex manner and react to changes by switching their phenotype and functional activation states. In order to understand the development of brain diseases, it is imperative to elucidate up- or down-regulation of genes involved in microglia polarisation in time-profile by a simple-to-use strategy. Here, we present a new imaging strategy to follow promoter activity of genes involved in microglia polarisation. We lentivirally transduced BV-2 microglia cells in culture with constructs consisting of the induced nitric oxide synthase (iNOS), Fc gamma receptor III (Fcgr3) (both resembling the pro-inflammatory M1-like phenotype) or Chitinase-like 3 (Chil3/Ym1) (resembling the anti-inflammatory M2-like phenotype) promoters and stimulated transgenic cells with potent activators for pro- or anti-inflammatory response, such as lipopolysaccharide (LPS) + interferon gamma (IFN-gamma) or interleukin (IL)-4, respectively. Promoter activities upon polarisation phases were quantitatively assessed by the two imaging reporters Luc2 for bioluminescence and eGFP for fluorescence.

Details

show
hide
Language(s):
 Dates: 2018-092018
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: Other: 29790106
DOI: 10.1007/s11481-018-9789-2
ISSN: 1557-1904 (Electronic)1557-1890 (Linking)
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: J Neuroimmune Pharmacol
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 13 (3) Sequence Number: - Start / End Page: 371 - 382 Identifier: -