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  Autophagy balances mtDNA synthesis and degradation by DNA polymerase POLG during starvation

Medeiros, T. C., Thomas, R. L., Ghillebert, R., & Graef, M. (2018). Autophagy balances mtDNA synthesis and degradation by DNA polymerase POLG during starvation. J Cell Biol. doi:10.1083/jcb.201801168.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-7215-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-73A7-E
Genre: Journal Article

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Medeiros, T. C.1, Author
Thomas, R. L.1, Author
Ghillebert, R.1, Author
Graef, M.1, Author
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1Graef – Effectors and Regulation of Autophagy during Ageing, Max Planck Research Groups, Max Planck Institute for Biology of Ageing, Max Planck Society, Joseph-Stelzmann-Str. 9b, D-50931 Cologne, DE, ou_1942295              

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 Abstract: Mitochondria contain tens to thousands of copies of their own genome (mitochondrial DNA [mtDNA]), creating genetic redundancy capable of buffering mutations in mitochondrial genes essential for cellular function. However, the mechanisms regulating mtDNA copy number have been elusive. Here we found that DNA synthesis and degradation by mtDNA polymerase gamma (POLG) dynamically controlled mtDNA copy number in starving yeast cells dependent on metabolic homeostasis provided by autophagy. Specifically, the continuous mtDNA synthesis by POLG in starving wild-type cells was inhibited by nucleotide insufficiency and elevated mitochondria-derived reactive oxygen species in the presence of autophagy dysfunction. Moreover, after prolonged starvation, 3'-5' exonuclease-dependent mtDNA degradation by POLG adjusted the initially increasing mtDNA copy number in wild-type cells, but caused quantitative mtDNA instability and irreversible respiratory dysfunction in autophagy-deficient cells as a result of nucleotide limitations. In summary, our study reveals that mitochondria rely on the homeostatic functions of autophagy to balance synthetic and degradative modes of POLG, which control copy number dynamics and stability of the mitochondrial genome.

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 Dates: 2018-03-082018
 Publication Status: Published in print
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 Identifiers: Other: 29519802
DOI: 10.1083/jcb.201801168
ISSN: 1540-8140 (Electronic)0021-9525 (Linking)
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Title: J Cell Biol
Source Genre: Journal
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