English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Cryo-EM structure of Neurospora crassa respiratory complex IV

Bausewein, T., Nussberger, S., & Kühlbrandt, W. (2019). Cryo-EM structure of Neurospora crassa respiratory complex IV. IUCrJ, 6(4), 773-780. doi:10.1107/S2052252519007486.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Bausewein, Thomas1, Author           
Nussberger, Stephan2, Author
Kühlbrandt, Werner1, Author                 
Affiliations:
1Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068291              
2Abteilung Biophysik, Institut für Biomaterialien und biomolekulare Systeme, Universität Stuttgart, Stuttgart, Germany, ou_persistent22              

Content

show
hide
Free keywords: 3D reconstruction and image processing; Neurospora crassa; automation; cryo-electron microscopy; cytochrome c oxidase; respiratory complex IV; single-particle cryo-EM; structure determination
 Abstract: In fungi, the mitochondrial respiratory chain complexes (complexes I-IV) are responsible for oxidative phosphorylation, as in higher eukaryotes. Cryo-EM was used to identify a 200 kDa membrane protein from Neurospora crassa in lipid nanodiscs as cytochrome c oxidase (complex IV) and its structure was determined at 5.5 Å resolution. The map closely resembles the cryo-EM structure of complex IV from Saccharomyces cerevisiae. Its ten subunits are conserved in S. cerevisiae and Bos taurus, but other transmembrane subunits are missing. The different structure of the Cox5a subunit is typical for fungal complex IV and may affect the interaction with complex III in a respiratory supercomplex. Additional density was found between the matrix domains of the Cox4 and Cox5a subunits that appears to be specific to N. crassa.

Details

show
hide
Language(s): eng - English
 Dates: 2019-02-212019-05-232019-06-26
 Publication Status: Published online
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1107/S2052252519007486
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: IUCrJ
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Chester CH1 2HU, England : International Union of Crystallography (IUCr)
Pages: - Volume / Issue: 6 (4) Sequence Number: - Start / End Page: 773 - 780 Identifier: ISSN: 2052-2525
CoNE: https://pure.mpg.de/cone/journals/resource/2052-2525