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  Qualitative and quantitative analysis of chemicals emitted from the pheromone gland of individual Heliothis subflexa females

Nojima, S., Classen, A., Groot, A. T., & Schal, C. (2018). Qualitative and quantitative analysis of chemicals emitted from the pheromone gland of individual Heliothis subflexa females. PLoS One, 13(8): e0202035. doi:10.1371/journal.pone.0202035.

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Nojima, Satoshi, Author
Classen, Alice, Author
Groot, Astrid T.1, Author           
Schal, Coby, Author
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1Extreme Timescales, Research Groups, Max Planck Research Department for Structural Dynamics, Department of Physics, University of Hamburg, External Organizations, Luruper Chaussee 149, 22761 Hamburg, Germany, DE, ou_2173639              

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 Abstract: The chemicals emitted from the sex pheromone gland of individual Heliothis subflexa females were sampled using a short section of thick-film megabore fused silica capillary column, and the pheromone glands of the same females were extracted after the effluvia collection. Both samples were treated with a silylation reagent, and then subjected to gas chromatography-chemical ionization-mass spectrometry for quantitative and qualitative analysis of all components. The total amount of all 11 components emitted from the glands of calling females was 153 ng/female/hr, which was substantially higher than previously reported. The ratios of the pheromone components in the volatile emissions and pheromone gland extracts were generally similar to previous studies, but with notable differences. The collections of volatiles and gland extractions contained, respectively: Z9-14:Ald (1.57%, 1.35%), 14:Ald (3.78%, 1.51%), Z7 + Z9-16:Ald (9.60%, 3.59%), Z11-16:Ald (76.14%, 18.94%), 16:Ald (2.95%, 2.17%), Z9-16:OH (0.07%, 7.21%), Z11-16:OH (1.11%, 49.04%), Z7-16:OAc (0.48%, 1.73%), Z9-16:OAc (1.32%, 4.02%), and Z11-16:OAc (2.98%, 10.43%). The thick-film megabore column is an efficient approach for sampling the headspace for semiochemicals.

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 Dates: 2018-07-262018-08-14
 Publication Status: Published online
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 Identifiers: Other: EXT719
DOI: 10.1371/journal.pone.0202035
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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 13 (8) Sequence Number: e0202035 Start / End Page: - Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850