English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Redox-regulation of photorespiration through mitochondrial thioredoxin o1

Reinholdt, O., Schwab, S., Zhang, Y., Reichheld, J.-P., Fernie, A. R., Hagemann, M., et al. (2019). Redox-regulation of photorespiration through mitochondrial thioredoxin o1. Plant Physiology, 181(2), 442-457. doi:10.1104/pp.19.00559.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0005-1030-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-1031-1
Genre: Journal Article

Files

show Files

Locators

show
hide
Locator:
Link (Any fulltext)
Description:
-

Creators

show
hide
 Creators:
Reinholdt, Ole1, Author
Schwab, Saskia1, Author
Zhang, YJ2, Author              
Reichheld, Jean-Philippe1, Author
Fernie, A. R.2, Author              
Hagemann, Martin1, Author
Timm, Stefan1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

Content

show
hide
Free keywords: -
 Abstract: Photorespiration sustains photosynthesis in the presence of oxygen due to rapid metabolization of 2-phosphoglycolate (2-PG), the major side-product of the oxygenase activity of Rubisco that also directly impedes carbon assimilation and allocation. Despite the fact that both the biochemical reactions and the underlying genetics are well characterized, information concerning the regulatory mechanisms which adjust photorespiratory flux is rare. Here, we studied the impact of mitochondrial-localized thioredoxin o1 (TRXo1) on photorespiratory metabolism. The characterization of an Arabidopsis (Arabidopsis thaliana) T-DNA insertional line (trxo1-1) revealed an increase in the stoichiometry of photorespiratory CO2 release and impaired glycine-to-serine turnover after a shift from high-to-low CO2 without changes in glycine decarboxylase (GDC) gene or protein expression. These effects were distinctly pronounced in a double mutant, where the TRXo1 mutation was combined with strongly reduced GDC T-protein expression. The double mutant (TxGT) showed reduced growth in air but not in high CO2, decreased photosynthesis and up to 54-fold more glycine alongside several redox-stress-related metabolites. Given that GDC proteins are potential targets for redox-regulation, we also examined the in vitro properties of recombinant GDC L-proteins (LPD) from plants and the cyanobacterium Synechocystis species strain PCC6803 and observed a redox-dependent inhibition by either artificial reducing agents or TRXo1 itself. Collectively, our results demonstrate that TRXo1 potentially adjusts photorespiration via redox-regulation of GDC in response to environmental changes.

Details

show
hide
Language(s): eng - English
 Dates: 20192019
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1104/pp.19.00559
BibTex Citekey: Reinholdtpp.00559.2019
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 181 (2) Sequence Number: - Start / End Page: 442 - 457 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438