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  Crystal structure of the glycoside hydrolase PssZ from Listeria monocytogenes

Wu, H., Shuai, Q., Li, D., Guo, L., Zhu, M., & Ma, L. Z. (2019). Crystal structure of the glycoside hydrolase PssZ from Listeria monocytogenes. Acta Crystallographica Section F: Structural Biology Communications, 75, 501-506. doi:10.1107/S2053230X19008100.

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 Creators:
Wu, Huijun1, Author
Shuai, Qiao2, Author              
Li, Defeng1, Author
Guo, Lu1, Author
Zhu, Meijun1, Author
Ma, Luyan Z.1, Author
Affiliations:
1external, ou_persistent22              
2Schulman, Brenda / Molecular Machines and Signaling, Max Planck Institute of Biochemistry, Max Planck Society, ou_2466699              

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Free keywords: BIOFILM FORMATION; RESISTANCE; SURFACES; PHENIXBiochemistry & Molecular Biology; Biophysics; Crystallography; Listeria monocytogenes; glycoside hydrolases; SAD phasing; PssZ; (alpha/alpha)(6)-barrel fold; biofilms;
 Abstract: Biofilms are microbial communities that are embedded in the extracellular matrix. The exopolysaccharide (EPS) is a key component of the biofilm matrix that maintains the structure of the biofilm and protects the bacteria from antimicrobials. Microbial glycoside hydrolases have been exploited to disrupt biofilms by breaking down EPSs. PssZ has recently been identified as a glycoside hydrolase that can disperse aggregates of Listeria monocytogenes. In this study, the crystal structure of PssZ has been determined at 1.6 angstrom resolution. PssZ belongs to glycoside hydrolase family 8 and adopts a classical (alpha/alpha)(6)-barrel fold. This architecture forms a deep groove which may serve as the substrate-binding pocket. The conserved catalytic residues (Glu72, Trp110, Asn119, Phe167, Tyr183 and Asp232) are localized at the centre of the groove. This crystal structure will help to improve the understanding of the hydrolytic mechanism of PssZ and its application as a biofilm disrupter.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000474470400005
DOI: 10.1107/S2053230X19008100
 Degree: -

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Title: Acta Crystallographica Section F: Structural Biology Communications
Source Genre: Journal
 Creator(s):
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Publ. Info: Blackwell Publishing Limited
Pages: - Volume / Issue: 75 Sequence Number: - Start / End Page: 501 - 506 Identifier: ISSN: 1744-3091
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000017210_1