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  High-resolution 3D light microscopy with STED and RESOLFT

Sahl, S. J., & Hell, S. W. (2019). High-resolution 3D light microscopy with STED and RESOLFT. In J. F. Bille (Ed.), High resolution imaging in microscopy and ophthalmology (pp. 3-32). Cham: Springer. doi:10.1007/978-3-030-16638-0_1.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0004-81DB-3 Version Permalink: https://hdl.handle.net/21.11116/0000-0004-EB95-B
Genre: Book Chapter

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Bille_2019_High-ResolutionImagingMicroscopyOphthalmology_3.pdf (Any fulltext), 4MB
 
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 Creators:
Sahl, Steffen J., Author
Hell, Stefan W.1, Author           
Affiliations:
1Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society, ou_2364730              

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Free keywords: Fluorescence imaging ; Superresolution ; Nanoscopy ; Molecular states : Diffraction-unlimited resolution ; Far-field optics ; Neuroscience ; Cell biology ; Stimulated emission ; Switchable proteins
 Abstract: This chapter discusses the simple yet powerful ideas which have allowed to break the diffraction resolution limit of lens-based optical microscopy. The basic principles and standard implementations of STED (stimulated emission depletion) and RESOLFT (reversible saturable/switchable optical linear (fluorescence) transitions) microscopy are introduced, followed by selected highlights of recent advances, including MINFLUX (minimal photon fluxes) nanoscopy with molecule-size (~1 nm) resolution.

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Language(s): eng - English
 Dates: 2019-08-142019
 Publication Status: Issued
 Pages: 30
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-3-030-16638-0_1
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Title: High resolution imaging in microscopy and ophthalmology
Source Genre: Book
 Creator(s):
Bille, Josef F., Editor
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Publ. Info: Cham : Springer
Pages: XX, 407 Volume / Issue: - Sequence Number: - Start / End Page: 3 - 32 Identifier: ISBN: 978-3-030-16637-3
ISBN: 978-3-030-16638-0