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  In situ detection of Escherichia coli cells containing ColE1-related plasmids by hybridization to regulatory RNA II

Juretschko, S., Schönhuber, W., Kulakauskas, S., Ehrlich, D., Schleifer, K., & Amann, R. I. (1999). In situ detection of Escherichia coli cells containing ColE1-related plasmids by hybridization to regulatory RNA II. Systematic and Applied Microbiology, 22(1), 1-8.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0005-47BA-A Version Permalink: https://hdl.handle.net/21.11116/0000-0005-47BB-9
Genre: Journal Article

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Schoenhuber_1999.pdf (Publisher version), 7MB
 
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Juretschko, S., Author
Schönhuber, Wilhelm1, Author           
Kulakauskas, S., Author
Ehrlich, D.S., Author
Schleifer, K.H., Author
Amann, Rudolf I.1, Author           
Affiliations:
1Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481696              

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 Abstract: A method is described for the in situ detection of individual whole fixed cells of Escherichia coli containing ColE1-related plasmids. It makes use of fluorescence in situ hybridization (FISH) and the regulatory RNA II as a target molecule for both, Cy3- and HRP-labeled olinucleotide probes. Various methods for signal amplification were compared. Probes targeting the regulatory RNA I did not result in the in situ detection of plasmid-bearing cells.

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Language(s): eng - English
 Dates: 1999
 Publication Status: Issued
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000079214500001
 Degree: -

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Title: Systematic and Applied Microbiology
  Other : System. Appl. Microbiol.
Source Genre: Journal
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Publ. Info: Stuttgart : Urban & Fischer
Pages: - Volume / Issue: 22 (1) Sequence Number: - Start / End Page: 1 - 8 Identifier: ISSN: 0723-2020
CoNE: https://pure.mpg.de/cone/journals/resource/954928582871