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  Application of 23S rDNA-targeted oligonucleotide probes specific for enterococci to water hygiene control

Frahm, E., Heiber, I., Hoffmann, S., Koob, C., Meier, H., Ludwig, W., et al. (1998). Application of 23S rDNA-targeted oligonucleotide probes specific for enterococci to water hygiene control. Systematic and Applied Microbiology, 21(3), 450-453. doi:10.1016/S0723-2020(98)80055-6.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0005-42A1-A Version Permalink: https://hdl.handle.net/21.11116/0000-0005-42A2-9
Genre: Journal Article

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Amann_1998.pdf (Publisher version), 3MB
 
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 Creators:
Frahm, E., Author
Heiber, I., Author
Hoffmann, S., Author
Koob, C., Author
Meier, H., Author
Ludwig, W., Author
Amann, Rudolf I.1, Author           
Schleifer, K.H., Author
Obst, U., Author
Affiliations:
1Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481696              

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 Abstract: Identification of enterococci species by hybridization with recently designed species-specific and group-specific 23S rDNA-targeted oligonucleotide probes was superior to results obtained with a common biochemical test panel. Considering these findings, a molecular biological procedure for the detection of enterococci in water samples was developed. A short enrichment is followed by an amplification step and a hybridization reaction in microtiter plate format. The detection limit is about 1 CFU/ml, and results are available within 26 h.

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Language(s): eng - English
 Dates: 1998
 Publication Status: Issued
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000076027900015
DOI: 10.1016/S0723-2020(98)80055-6
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Title: Systematic and Applied Microbiology
  Other : System. Appl. Microbiol.
Source Genre: Journal
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Publ. Info: Stuttgart : Urban & Fischer
Pages: - Volume / Issue: 21 (3) Sequence Number: - Start / End Page: 450 - 453 Identifier: ISSN: 0723-2020
CoNE: https://pure.mpg.de/cone/journals/resource/954928582871