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  Genetic diversity and expression of the [NiFe] hydrogenase large-subunit gene of Desulfovibrio spp. in environmental samples

Wawer, C., Jetten, M., & Muyzer, G. (1997). Genetic diversity and expression of the [NiFe] hydrogenase large-subunit gene of Desulfovibrio spp. in environmental samples. Applied and Environmental Microbiology, 63(11), 4360-4369.

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 Urheber:
Wawer, Cathrin1, Autor           
Jetten, M.S.M., Autor
Muyzer, Gerad1, Autor           
Affiliations:
1Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481696              

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 Zusammenfassung: The genetic diversity and expression of the [NiFe] hydrogenase large-subunit gene of Desulfovibrio spp. in environmental samples were determined in order to show in parallel the existing and active members of Desulfovibrio populations. DNA and total RNA were extracted from different anaerobic bioreactor samples; RNA was transcribed into cDNA. Subsequently, PCR was performed to amplify a ca.-440-bp fragment of the [NiFe] hydrogenase large-subunit gene and its mRNA Denaturing gradient gel electrophoresis analysis was used to separate the PCR products according to their sequence and thereby to visualize the individual community members. Desulfovibrio strains corresponding to amplified [NiFe] hydrogenase transcripts were regarded as metabolically active, because in pure cultures transcripts were detectable in exponentially growing cells but not in cultures in the stationary phase. DNA sequencing and comparative sequence analysis were used to identify the detected organisms on the basis of their [NiFe] hydrogenase sequences. The genes of characterized Desulfovibrio spp. showed a considerable extent of divergence (ca. 30%), whereas sequences obtained from bacterial populations of the bioreactors showed a low level of variation and indicated the coexistence of closely related strains probably belonging to the species Desulfovibrio sulfodismutans. Under methanogenic conditions, all detected populations were active; under denitrifying conditions, no [NiFe] hydrogenase mRNA was visible. Changes in activity and composition of Desulfovibrio populations caused by changes in the environmental conditions could be monitored by using the approach described in this study.

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Sprache(n): eng - English
 Datum: 1997
 Publikationsstatus: Erschienen
 Seiten: 10
 Ort, Verlag, Ausgabe: -
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 Identifikatoren: ISI: A1997YE28100033
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Titel: Applied and Environmental Microbiology
  Andere : Appl. Environ. Microbiol.
Genre der Quelle: Zeitschrift
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Affiliations:
Ort, Verlag, Ausgabe: American Society for Microbiology (ASM)
Seiten: - Band / Heft: 63 (11) Artikelnummer: - Start- / Endseite: 4360 - 4369 Identifikator: ISSN: 0099-2240
CoNE: https://pure.mpg.de/cone/journals/resource/954927519600