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  Use of green fluorescent protein as a marker for ecological studies of activated sludge communities

Eberl, L., Schulze, R., Ammendola, A., Geisenberger, O., Erhart, R., Sternberg, C., et al. (1997). Use of green fluorescent protein as a marker for ecological studies of activated sludge communities. FEMS Microbiology Letters, 149(1), 77-83. doi:10.1016/S0378-1097(97)00058-X.

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Amann_1997.pdf (Publisher version), 533KB
 
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Eberl, L, Author
Schulze, R, Author
Ammendola, A, Author
Geisenberger, O, Author
Erhart, R, Author
Sternberg, C, Author
Molin, S, Author
Amann, Rudolf I.1, Author           
Affiliations:
1Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481696              

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 Abstract: The potential of the green fluorescent protein (GFP) as a marker gene for ecological investigations of an activated sludge community was assessed. By inserting the hybrid transposon mini-Tn5 gfp into the chromosome of Pseudomonas putida KT2442 a strongly fluorescent mutant was obtained. This strain was used for in vivo tracking of individual cells after introduction into a simple sludge microcosm. It is demonstrated that the observed reduction of introduced bacteria from sewage is mainly the result of predation by protozoa. The feasibility of combining detection of GFP fluorescence with whole cell hybridization employing fluorescently labeled, rRNA-targeted oligonucleotides in paraformaldehyde fixed samples is demonstrated.

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Language(s): eng - English
 Dates: 1997
 Publication Status: Issued
 Pages: 7
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Title: FEMS Microbiology Letters
  Other : FEMS Microbiol. Lett.
Source Genre: Journal
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Publ. Info: Amsterdam : No longer published by Elsevier
Pages: - Volume / Issue: 149 (1) Sequence Number: - Start / End Page: 77 - 83 Identifier: ISSN: 0378-1097
CoNE: https://pure.mpg.de/cone/journals/resource/954925484705