English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Distribution of bacterial populations in a stratified fjord (Mariager fjord, Denmark) quantified by in situ hybridization and related to chemical gradients in the water column

Ramsing, N., Fossing, H., Ferdelman, T. G., Andersen, F., & Thamdrup, B. (1996). Distribution of bacterial populations in a stratified fjord (Mariager fjord, Denmark) quantified by in situ hybridization and related to chemical gradients in the water column. Applied and Environmental Microbiology, 62(10), 3915-3915.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
Ramsing_1996.pdf (Publisher version), 576KB
Name:
Ramsing_1996.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Ramsing, N.B.1, Author              
Fossing, Hendrik2, Author              
Ferdelman, Timothy G.2, Author              
Andersen, F., Author
Thamdrup, B.2, Author              
Affiliations:
1Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, Celsiusstraße 1, 28359 Bremen, DE, ou_2481696              
2Department of Biogeochemistry, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481693              

Content

show
hide
Free keywords: -
 Abstract: The vertical distribution of major and intermediate electron accepters and donors was measured in a shallow stratified fjord. Peaks of zero valence sulfur, Mn(TV), and Fe(III) were observed in the chemocline separating oxic surface waters from sulfidic and anoxic bottom waters. The vertical fluxes of electron accepters and donors (principally O-2 and H2S) balanced within 5%; however, the zones of oxygen reduction and sulfide oxidation were clearly separated. The pathway of electron transfer between O-2, and H2S was not apparent from the distribution of sulfur, nitrogen, or metal compounds investigated. The chemical zonation was related to bacterial populations as detected by ethidium bromide (EtBr) staining and by in situ hybridization with fluorescent oligonucleotide probes of increasing specificity. About half of all EtBr-stained cells were detectable with a general oligonucleotide probe for all eubacteria when digital image analysis algorithms were used to improve sensitivity. Both EtBr staining and hybridization indicated a surprisingly uniform distribution of bacteria throughout the water column. However, the average cell size and staining intensity as well as the abundance of different morphotypes changed markedly within the chemocline. The constant overall cell counts thus concealed pronounced population shifts within the water column. Cells stained with a delta 385 probe (presumably sulfate-reducing bacteria) were detected at the chemocline at about 5 x 10(5) cells per ml, and this concentration increased to 2 x 10(5) cells per ml beneath the chemocline. A long slim rod-shaped bacterium was found in large numbers in the oxic part of the chemocline, whereas large ellipsoid cells dominated at greater depth. Application of selective probes for known genera of sulfate-reducing bacteria gave only low cell counts, and thus it was not possible to identify the dominant morphotypes of the sulfate-reducing community.

Details

show
hide
Language(s): eng - English
 Dates: 1996
 Publication Status: Published in print
 Pages: 16
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: A1996UD95000042
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Applied and Environmental Microbiology
  Other : Appl. Environ. Microbiol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: American Society for Microbiology (ASM)
Pages: - Volume / Issue: 62 (10) Sequence Number: - Start / End Page: 3915 - 3915 Identifier: ISSN: 0099-2240
CoNE: https://pure.mpg.de/cone/journals/resource/954927519600