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  Proteome Analysis of Enriched Heterocysts from Two Hydrogenase Mutants from Anabaena sp. PCC 7120

Kourpa, K., Manarolaki, E., Lyratzakis, A., Strataki, V., Rupprecht, F. A., Langer, J. D., et al. (2019). Proteome Analysis of Enriched Heterocysts from Two Hydrogenase Mutants from Anabaena sp. PCC 7120. Proteomics, 19(19): 1800332. doi:10.1002/pmic.201800332.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-A0A7-A Version Permalink: http://hdl.handle.net/21.11116/0000-0004-DA38-8
Genre: Journal Article
Alternative Title : Proteomics

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 Creators:
Kourpa, Katerina1, Author
Manarolaki, Eftychia1, Author
Lyratzakis, Alexandros1, Author
Strataki, Vasso1, Author
Rupprecht, Fiona A.2, Author              
Langer, Julian David2, 3, Author              
Tsiotis, Georgios1, Author
Affiliations:
1Division of Biochemistry, Department of Chemistry, University of Crete, GR-71003 Voutes, Greece, ou_persistent22              
2Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society, ou_2461710              
3Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              

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Free keywords: anabaena sp. PCC 7120; cyanobacteria; heterocysts; hydrogenase mutant; proteome
 Abstract: Cyanobacteria are oxygenic photosynthetic prokaryotes and play a crucial role in the Earth's carbon and nitrogen cycles. The photoautotrophic cyanobacterium Anabaena sp. PCC 7120 has the ability to fix atmospheric nitrogen in heterocysts and produce hydrogen as a byproduct through a nitrogenase. In order to improve hydrogen production, mutants from Anabaena sp. PCC 7120 are constructed by inactivation of the uptake hydrogenase (ΔhupL) and the bidirectional hydrogenase (ΔhoxH) in previous studies. Here the proteomic differences of enriched heterocysts between these mutants cultured in N2-fixing conditions are investigated. Using a label-free quantitative proteomics approach, a total of 2728 proteins are identified and it is found that 79 proteins are differentially expressed in the ΔhupL and 117 proteins in the ΔhoxH variant. The results provide for the first time comprehensive information on proteome regulation of the uptake hydrogenase and the bidirectional hydrogenase, as well as systematic data on the hydrogen related metabolism in Anabaena sp. PCC 7120.

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Language(s): eng - English
 Dates: 2019-07-122018-09-032019-08-202019-08-20
 Publication Status: Published online
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: Proteomics
Source Genre: Journal
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Publ. Info: Weinheim : WILEY-VCH
Pages: - Volume / Issue: 19 (19) Sequence Number: 1800332 Start / End Page: - Identifier: ISSN: 1615-9853
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000294310