English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Dynamic tuning of FRET in a green fluorescent protein biosensor.

Trigo-Mourino, P., Thestrup, T., Griesbeck, O., Griesinger, C., Becker, S., & S (2019). Dynamic tuning of FRET in a green fluorescent protein biosensor. Science Advances, 5(8): eaaw4988. doi:10.1126/sciadv.aaw4988.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0004-95FE-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-9602-0
Genre: Journal Article

Files

show Files
hide Files
:
3157905.pdf (Publisher version), 2MB
Name:
3157905.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3157905_Suppl.htm (Supplementary material), 228KB
Name:
3157905_Suppl.htm
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/xhtml+xml / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Trigo-Mourino, P.1, Author              
Thestrup, T., Author
Griesbeck, O., Author
Griesinger, C.1, Author              
Becker, S.1, Author              
S, Author
Affiliations:
1Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

Content

show
hide
Free keywords: -
 Abstract: Forster resonance energy transfer (FRET) between mutants of green fluorescent protein is widely used to monitor protein-protein interactions and as a readout mode in fluorescent biosensors. Despite the fundamental importance of distance and molecular angles of fluorophores to each other, structural details on fluorescent protein FRET have been missing. Here, we report the high-resolution x-ray structure of the fluorescent proteins mCerulean3 and cpVenus within the biosensor Twitch-2B, as they undergo FRET and characterize the dynamics of this biosensor with B-0(2)-dependent paramagnetic nuclear magnetic resonance at 900 MHz and 1.1 GHz. These structural data provide the unprecedented opportunity to calculate FRET from the x-ray structure and to compare it to experimental data in solution. We find that interdomain dynamics limits the FRET effect and show that a rigidification of the sensor further enhances FRET.

Details

show
hide
Language(s): eng - English
 Dates: 2019-08-07
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1126/sciadv.aaw4988
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Science Advances
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 9 Volume / Issue: 5 (8) Sequence Number: eaaw4988 Start / End Page: - Identifier: -