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  Structural characterization and decontamination of dental calculus for ancient starch research

Soto, M., Inwood, J., Clarke, S., Crowther, A., Covelli, D., Favreau, J., et al. (2019). Structural characterization and decontamination of dental calculus for ancient starch research. Archaeological and Anthropological Sciences, 11(9), 4847-4872. doi:10.1007/s12520-019-00830-7.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-9A63-F Version Permalink: http://hdl.handle.net/21.11116/0000-0004-9A64-E
Genre: Journal Article

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 Creators:
Soto, Maria, Author
Inwood, Jamie, Author
Clarke, Siobhán, Author
Crowther, Alison1, Author              
Covelli, Danielle, Author
Favreau, Julien, Author
Itambu, Makarius, Author
Larter, Steve, Author
Lee, Patrick, Author
Lozano, Marina, Author
Maley, Jason, Author
Mwambwiga, Aloyce, Author
Patalano, Robert, Author
Sammynaiken, Ramaswami, Author
Vergès, Josep M., Author
Zhu, Jianfeng, Author
Mercader, Julio, Author
Affiliations:
1Archaeology, Max Planck Institute for the Science of Human History, Max Planck Society, ou_2074312              

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Free keywords: Structural chemical characterization . Raman . XPS . P-XRD . Ancient dental calculus . Ancient starch research . Decontamination prior to decalcification . Starch contamination
 Abstract: Ancient dental calculus research currently relies on destructive techniques whereby archeological specimens are broken down to determine their contents. Two strategies that could partly remediate a permanent loss of the original sample and enhance future analysis and reproducibility include (1) structural surface characterization through spectroscopy along with crystallographic and spectroscopic analysis of its molecular structure, and (2) surface decontamination protocols in which the efficacy of cleaning dental calculus prior to extraction is demonstrated. Dental calculus provides ancient starch research a niche where granules may be adsorbed to minerals, coated, overgrown, entrapped, and/or protected from chemical degradation. While encapsulation offers protection from degradation, it does not shield the sample's surface from contamination. The most common approach to retrieving microbotanical particles from archeological calculus has been the direct decalcification of the sample, after a cleaning stage variously consisting of immersion in water, acids, and mechanical dislodgment via gas, sonication, and/or toothbrushes. Little is known about the efficiency of these methods for a complete removal of sediment/soil and unrelated microbotanical matter. In this paper, controlled laboratory experimentation leads to chemical structural characterization and a decontamination protocol to eradicate starch granules. Several concentrations of acids, bases, and enzymes were tested at intervals to understand their potential to gelatinize and fully destroy starch granules; arriving at a procedure that effectively eradicates modern starch prior to dissolution without damaging the matrix or entrapped starch microremains. This is the first attempt at creating synthetic calculus to understand and systematically test effective decontamination protocols for ancient starch research.

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Language(s): eng - English
 Dates: 2019-04-112019-09
 Publication Status: Published in print
 Pages: 26
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1007/s12520-019-00830-7
Other: shh2391
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Title: Archaeological and Anthropological Sciences
  Other : Archaeol Anthropol Sci
Source Genre: Journal
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Publ. Info: Berlin [u.a.] : Springer
Pages: - Volume / Issue: 11 (9) Sequence Number: - Start / End Page: 4847 - 4872 Identifier: ISSN: 1866-9557
CoNE: https://pure.mpg.de/cone/journals/resource/1866-9557