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  Tenogenic properties of mesenchymal progenitor cells are compromised in an inflammatory environment

Brandt, L., Schubert, S., Scheibe, P., Brehm, W., Franzen, J., Gross, C., et al. (2018). Tenogenic properties of mesenchymal progenitor cells are compromised in an inflammatory environment. International Journal of Molecular Sciences, 19(9): 2549. doi:10.3390/ijms19092549.

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 Creators:
Brandt, Luisa1, Author
Schubert, Susanna1, 2, Author
Scheibe, Patrick1, Author              
Brehm, Walter1, 3, Author
Franzen, Jan1, 2, Author
Gross, Claudia1, Author
Burk, Janina1, 2, 4, Author
Affiliations:
1Saxonian Incubator for Clinical Translation (SIKT), University of Leipzig, Germany, ou_persistent22              
2Institute of Veterinary Physiology, University of Leipzig, Germany, ou_persistent22              
3Department for Horses, Faculty of Veterinary Medicine, University of Leipzig, Germany, ou_persistent22              
4Institute of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria, ou_persistent22              

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Free keywords: Mesenchymal stromal cells (MSC); ASC; Tendon; Extracellular matrix; Bioreactor; Inflammation; Interleukin-1 (IL-1); Tumor necrosis factor-α (TNF-α); Leukocytes; Co-culture
 Abstract: Transplantation of multipotent mesenchymal progenitor cells is a valuable option for treating tendon disease. Tenogenic differentiation leading to cell replacement and subsequent matrix modulation may contribute to the regenerative effects of these cells, but it is unclear whether this occurs in the inflammatory environment of acute tendon disease. Equine adipose-derived stromal cells (ASC) were cultured as monolayers or on decellularized tendon scaffolds in static or dynamic conditions, the latter represented by cyclic stretching. The impact of different inflammatory conditions, as represented by supplementation with interleukin-1β and/or tumor necrosis factor-α or by co-culture with allogeneic peripheral blood leukocytes, on ASC functional properties was investigated. High cytokine concentrations increased ASC proliferation and osteogenic differentiation, but decreased chondrogenic differentiation and ASC viability in scaffold culture, as well as tendon scaffold repopulation, and strongly influenced musculoskeletal gene expression. Effects regarding the latter differed between the monolayer and scaffold cultures. Leukocytes rather decreased ASC proliferation, but had similar effects on viability and musculoskeletal gene expression. This included decreased expression of the tenogenic transcription factor scleraxis by an inflammatory environment throughout culture conditions. The data demonstrate that ASC tenogenic properties are compromised in an inflammatory environment, with relevance to their possible mechanisms of action in acute tendon disease.

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Language(s): eng - English
 Dates: 2018-08-222018-07-312018-08-232018-08-282018-08
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.3390/ijms19092549
PMID: 30154348
PMC: PMC6163784
BibTex Citekey: Brandt2018
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Grant ID : BU 3110/1-1
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Funding organization : German Research Foundation (DFG)
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Funding organization : Saxon Ministry of Science and the Fine Arts
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Funding program : Open Access Publishing
Funding organization : University of Leipzig

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Title: International Journal of Molecular Sciences
  Abbreviation : Int. J. Mol. Sci.
Source Genre: Journal
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Publ. Info: Basel, Switzerland : MDPI AG
Pages: - Volume / Issue: 19 (9) Sequence Number: 2549 Start / End Page: - Identifier: ISSN: 1422-0067
CoNE: https://pure.mpg.de/cone/journals/resource/1422-0067