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  In vitro fusion of single synaptic and dense core vesicles reproduces key physiological properties.

Kreutzberger, A. J. B., Kiessling, V., Stroupe, C., Liang, B., Preobraschenski, J., Ganzella, M., et al. (2019). In vitro fusion of single synaptic and dense core vesicles reproduces key physiological properties. Nature Communications, 10: 3904. doi:10.1038/s41467-019-11873-8.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-AAFD-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-AB02-9
Genre: Journal Article

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 Creators:
Kreutzberger, A. J. B., Author
Kiessling, V., Author
Stroupe, C., Author
Liang, B., Author
Preobraschenski, J.1, Author              
Ganzella, M., Author
Kreutzberger, M. A. B., Author
Nakamoto, R., Author
Jahn, R.2, Author              
Castle, J. D., Author
Tamm, L. K., Author
Affiliations:
1Department of Neurobiology, MPI for biophysical chemistry, Max Planck Society, ou_578595              
2Laboratory of Neurobiology, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_3049887              

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 Abstract: Regulated exocytosis of synaptic vesicles is substantially faster than of endocrine dense core vesicles despite similar molecular machineries. The reasons for this difference are unknown and could be due to different regulatory proteins, different spatial arrangements, different vesicle sizes, or other factors. To address these questions, we take a reconstitution approach and compare regulated SNARE-mediated fusion of purified synaptic and dense core chromaffin and insulin vesicles using a single vesicle-supported membrane fusion assay. In all cases, Munc18 and complexin are required to restrict fusion in the absence of calcium. Calcium triggers fusion of all docked vesicles. Munc13 (C1C2MUN domain) is required for synaptic and enhanced insulin vesicle fusion, but not for chromaffin vesicles, correlating inversely with the presence of CAPS protein on purified vesicles. Striking disparities in calcium-triggered fusion rates are observed, increasing with curvature with time constants 0.23 s (synaptic vesicles), 3.3 s (chromaffin vesicles), and 9.1 s (insulin vesicles) and correlating with rate differences in cells.

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Language(s): eng - English
 Dates: 2019-08-29
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41467-019-11873-8
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Title: Nature Communications
Source Genre: Journal
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Pages: 11 Volume / Issue: 10 Sequence Number: 3904 Start / End Page: - Identifier: -