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  Standardized microgel beads as elastic cell mechanical probes

Girardo, S., Traeber, N., Wagner, K., Cojoc, G., Herold, C., Goswami, R., et al. (2018). Standardized microgel beads as elastic cell mechanical probes. JOURNAL OF MATERIALS CHEMISTRY B, 6(39), 6245-6261. doi:10.1039/c8tb01421c.

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Datensatz-Permalink: https://hdl.handle.net/21.11116/0000-0004-AF36-B Versions-Permalink: https://hdl.handle.net/21.11116/0000-0004-AF37-A
Genre: Zeitschriftenartikel

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 Urheber:
Girardo, S.1, Autor
Traeber, N.1, Autor
Wagner, K.1, Autor
Cojoc, G.1, Autor
Herold, C.1, Autor
Goswami, R.1, Autor
Schluessler, R.1, Autor
Abuhattum, S.1, Autor
Taubenberger, A.1, Autor
Reichel, F.1, Autor
Mokbel, D.1, Autor
Herbig, M.1, Autor
Schuermann, M.1, Autor
Mueller, P.1, Autor
Heida, T.1, Autor
Jacobi, A.1, Autor
Ulbricht, E.1, Autor
Thiele, J.1, Autor
Werner, C.1, Autor
Guck, Jochen2, Autor           
Affiliations:
1external, ou_persistent22              
2External Organizations, ou_persistent22              

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Schlagwörter: -
 Zusammenfassung: Cell mechanical measurements are gaining increasing interest in biological and biomedical studies. However, there are no standardized calibration particles available that permit the cross-comparison of different measurement techniques operating at different stresses and time-scales. Here we present the rational design, production, and comprehensive characterization of poly-acrylamide (PAAm) microgel beads mimicking size and overall mechanics of biological cells. We produced mono-disperse beads at rates of 20-60 kHz by means of a microfluidic droplet generator, where the pre-gel composition was adjusted to tune the beads' elasticity in the range of cell and tissue relevant mechanical properties. We verified bead homogeneity by optical diffraction tomography and Brillouin microscopy. Consistent elastic behavior of microgel beads at different shear rates was confirmed by AFM-enabled nanoindentation and real-time deformability cytometry (RT-DC). The remaining inherent variability in elastic modulus was rationalized using polymer theory and effectively reduced by sorting based on forward-scattering using conventional flow cytometry. Our results show that PAAm microgel beads can be standardized as mechanical probes, to serve not only for validation and calibration of cell mechanical measurements, but also as cell-scale stress sensors.

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Sprache(n): eng - English
 Datum: 2018
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: DOI: 10.1039/c8tb01421c
 Art des Abschluß: -

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Titel: JOURNAL OF MATERIALS CHEMISTRY B
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND : ROYAL SOC CHEMISTRY
Seiten: - Band / Heft: 6 (39) Artikelnummer: - Start- / Endseite: 6245 - 6261 Identifikator: ISSN: 2050-750X