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  Liquid application method for time-resolved analyses by serial synchrotron crystallography

Mehrabi, P., Schulz, E.-C., Agthe, M., Horrell, S., Bourenkov, G., von Stetten, D., et al. (2019). Liquid application method for time-resolved analyses by serial synchrotron crystallography. Nature methods, 16(10), 979-982. doi:10.1038/s41592-019-0553-1.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-ADB5-D Version Permalink: http://hdl.handle.net/21.11116/0000-0004-D998-C
Genre: Journal Article

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https://dx.doi.org/10.1038/s41592-019-0553-1 (Publisher version)
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 Creators:
Mehrabi, P.1, Author              
Schulz, E.-C.1, 2, Author              
Agthe, M.3, Author
Horrell, S.3, Author
Bourenkov, G.4, Author
von Stetten, D.4, Author
Leimkohl, J.-P.5, Author              
Schikora, H.5, Author              
Schneider, T. R.4, Author
Pearson, A. R.2, 3, Author
Tellkamp, F.5, Author              
Miller, R. J. D.1, 2, 6, Author              
Affiliations:
1Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
2Centre for Ultrafast Imaging, Universität Hamburg, ou_persistent22              
3Institute for Nanostructure and Solid State Physics, Universität Hamburg, ou_persistent22              
4European Molecular Biology Laboratory, Hamburg Unit c/o Deutsches Elektronen Synchrotron, ou_persistent22              
5Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2074322              
6University of Toronto, Departments of Chemistry and Physics, ou_persistent22              

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 Abstract: We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.

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Language(s): eng - English
 Dates: 2019-02-112019-08-062019-09-162019
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41592-019-0553-1
 Degree: -

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Project name : The authors thank D. Oberthuer (CFEL, DESY Hamburg) for the provision of the GlcNAc3 substrate immediately before data collection. The LAMA TR-SSX data were collected at beamline P14 and P14-2 (T-REXX) operated by EMBL Hamburg at the PETRA III storage ring (DESY, Hamburg, Germany). Construction of T-REXX was funded by the BMBF (Verbund Forschungsprojekt 05K16GU1). The authors are grateful to R. Dousza, L. Bultema and J. Besaw for their technical assistance during data collection. T-REXX beamtime was awarded as part of the EMBL BAG MX-660. The authors gratefully acknowledge the support provided by the Max Planck Society and the excellence cluster ‘The Hamburg Centre for Ultrafast Imaging—Structure, Dynamics and Control of Matter at the Atomic Scale’ of the Deutsche Forschungsgemeinschaft EXC 1074 project ID 194651731 (R.J.D.M.). P.M. was supported by the Alexander von Humboldt-Stiftung for postdoctoral researchers.
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Title: Nature methods
  Other : Nature methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: 4 Volume / Issue: 16 (10) Sequence Number: - Start / End Page: 979 - 982 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556