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  Proteomic mapping by rapamycin-dependent targeting of APEX2 identifies binding partners of VAPB at the inner nuclear membrane.

James, C., Müller, M., Goldberg, M. W., Lenz, C., Urlaub, H., & Kehlenbach, R. H. (2019). Proteomic mapping by rapamycin-dependent targeting of APEX2 identifies binding partners of VAPB at the inner nuclear membrane. Journal of Biological Chemistry, (in press). doi:10.1074/jbc.RA118.007283.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-AF80-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-AF84-2
Genre: Journal Article

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 Creators:
James, C., Author
Müller, M., Author
Goldberg, M. W., Author
Lenz, C.1, Author              
Urlaub, H.1, Author              
Kehlenbach, R. H., Author
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society, ou_578613              

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Free keywords: APEX; RAPIDS; VAPB; biotin; emerin; endoplasmic reticulum (ER); nuclear envelope; nuclear pore; peroxidase; proteomic proximity mapping
 Abstract: VAPB (vesicle-associated membrane protein-associated protein B) is a tail-anchored protein that is present at several contact sites of the endoplasmic reticulum (ER). We now show by immunoelectron microscopy that VAPB also localizes to the inner nuclear membrane (INM). Using a modified APEX2 (enhanced ascorbate peroxidase 2)-approach with rapamycin-dependent targeting of the peroxidase to a protein of interest, we searched for proteins that are in close proximity to VAPB, particularly at the INM. In combination with stable isotope labeling with amino acids in cell culture (SILAC), we confirmed many well-known interaction partners at the level of the ER with a clear distinction between specific and non-specific hits. Furthermore, we identified emerin, TMEM43 and ELYS as potential interaction partners of VAPB at the INM and the nuclear pore complex, respectively.

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Language(s): eng - English
 Dates: 2019-09-13
 Publication Status: Published online
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1074/jbc.RA118.007283
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Title: Journal of Biological Chemistry
Source Genre: Journal
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