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  A simplified procedure for the isolation of bacterial polypeptide elongation factor EF-Tu

Leberman, R., Antonsson, B., Giovanelli, R., Guariguata, R. A., Schumann, R., & Wittinghofer, A. (1980). A simplified procedure for the isolation of bacterial polypeptide elongation factor EF-Tu. Analytical Biochemistry, 104(1), 29-36. doi:10.1016/0003-2697(80)90272-9.

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AnalytBiochem_104_1980_29.pdf (Any fulltext), 3MB
 
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 Creators:
Leberman, Reuben1, Author           
Antonsson , B., Author
Giovanelli, Ruth1, Author           
Guariguata, Rafael A.1, Author           
Schumann, Renate2, Author           
Wittinghofer, Alfred3, Author           
Affiliations:
1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              
2Dietmar Manstein Group, Max Planck Institute for Medical Research, Max Planck Society, ou_1497708              
3Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              

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 Abstract: Polypeptide elongation factor EF-Tu can be isolated from bacterial cell extracts in two fractionation steps. The first is ion-exchange chromatography on DEAE-Sepharose, CL-6B, and the second is gel filtration on AcA 44. The method is illustrated with extracts from Escherichia coli, Bacillus stearothermophilus, and the thermophilic bacterium PS3. The extracts were obtained from lysozyme-treated cells and were processed without high-speed centrifugation or ammonium sulfate fractionation. The procedure is simple and rapid, gives higher yields than previous methods, and is easily scaled to any size preparation. The procedure also produces fractions enriched in the other polypeptide elongation factors EF-Ts and EF-G.

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Language(s): eng - English
 Dates: 1979-07-112004-11-191980-05-01
 Publication Status: Published in print
 Pages: 8
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 Rev. Type: Peer
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Title: Analytical Biochemistry
Source Genre: Journal
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Publ. Info: San Diego, CA : Academic Press
Pages: - Volume / Issue: 104 (1) Sequence Number: - Start / End Page: 29 - 36 Identifier: ISSN: 0003-2697
CoNE: https://pure.mpg.de/cone/journals/resource/954922644003