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  Morpho-Rheological Fingerprinting of Rod Photoreceptors Using Real-Time Deformability Cytometry

Santos-Ferreira, T., Herbig, M., Otto, O., Carido, M., Karl, M. O., Michalakis, S., et al. (2019). Morpho-Rheological Fingerprinting of Rod Photoreceptors Using Real-Time Deformability Cytometry. Cytometry A, 95(11), 1145-1157. doi:10.1002/cyto.a.23798.

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Santos-Ferreira_et_al-2019-Cytometry_Part_A.pdf (Publisher version), 9MB
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Santos-Ferreira_et_al-2019-Cytometry_Part_A.pdf
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© 2019 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
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 Creators:
Santos-Ferreira, Tiago1, Author
Herbig, Maik1, Author
Otto, Oliver1, Author
Carido, Madalena1, Author
Karl, Mike O.1, Author
Michalakis, Stylianos1, Author
Guck, Jochen2, 3, Author           
Ader, Marius1, Author
Affiliations:
1external, ou_persistent22              
2Guck Division, Max Planck Institute for the Science of Light, Max Planck Society, ou_3164416              
3Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society, ou_3164414              

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Free keywords: morpho-mechanical properties; retina; photoreceptor; embryonic stem cell
 Abstract: Distinct cell-types within the retina are mainly specified by morphological and molecular parameters, however, physical properties are increasingly recognized as a valuable tool to characterize and distinguish cells in diverse tissues. High-throughput analysis of morpho-rheological features has recently been introduced using real-time deformability cytometry (RT-DC) providing new insights into the properties of different cell-types. Rod photoreceptors represent the main light sensing cells in the mouse retina that during development forms apically the densely packed outer nuclear layer. Currently, enrichment and isolation of photoreceptors from retinal primary tissue or pluripotent stem cell-derived organoids for analysis, molecular profiling, or transplantation is achieved using flow cytometry or magnetic activated cell sorting approaches. However, such purification methods require genetic modification or identification of cell surface binding antibody panels. Using primary retina and embryonic stem cell-derived retinal organoids, we characterized the inherent morpho-mechanical properties of mouse rod photoreceptors during development based on RT-DC. We demonstrate that rods become smaller and more compliant throughout development and that these features are suitable to distinguish rods within heterogenous retinal tissues. Hence, physical properties should be considered as additional factors that might affect photoreceptor differentiation and retinal development besides representing potential parameters for label-free sorting of photoreceptors.

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Language(s): eng - English
 Dates: 2019-05-062019-05-20
 Publication Status: Published online
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/cyto.a.23798
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Title: Cytometry A
Source Genre: Journal
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Publ. Info: Wiley Online Library
Pages: - Volume / Issue: 95 (11) Sequence Number: - Start / End Page: 1145 - 1157 Identifier: -