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  Measurement of angstrom to nanometer molecular distances with 19F nuclear spins by EPR/ENDOR spectroscopy.

Bennati, M., Meyer, A., Dechert, S., Dey, S., & Höbartner, C. (2020). Measurement of angstrom to nanometer molecular distances with 19F nuclear spins by EPR/ENDOR spectroscopy. Angewandte Chemie International Edition, 59(1), 373-379. doi:10.1002/anie.201908584.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-B952-F Version Permalink: http://hdl.handle.net/21.11116/0000-0005-937B-B
Genre: Journal Article

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 Creators:
Bennati, M.1, Author              
Meyer, A.1, Author              
Dechert, S., Author
Dey, S., Author
Höbartner, C., Author
Affiliations:
1Research Group of Electron Paramagnetic Resonance, MPI for Biophysical Chemistry, Max Planck Society, ou_578606              

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Free keywords: RNA; Structural biology; fluorine; high field ENDOR; spin labelling
 Abstract: Spectroscopic and biophysical methods for structural determination at atomic resolution are fundamental in studies of biological function. Here we introduce an approach to measure molecular distances in bio-macromolecules using 19 F nuclear spins and nitroxide radicals in combination with high-frequency (94 GHz/ 3.4 Tesla) electron-nuclear double resonance (ENDOR). The small size and large gyromagnetic ratio of the 19 F label enables to access distances up to about 1.5 nm with accuracy 0.1 - 1 Å. The experiment is not limited by the size of the bio-macromolecule. Performance is illustrated on synthesized, fluorinated model compounds as well as spin-labelled RNA duplexes. The results demonstrate that our simple but strategic spin labelling procedure combined with state-of-the-art spectroscopy accesses a distance range crucial to elucidate active sites of nucleic acids or proteins in solution state.

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Language(s): eng - English
 Dates: 2019-09-202020-01-02
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1002/anie.201908584
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Title: Angewandte Chemie International Edition
Source Genre: Journal
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Pages: - Volume / Issue: 59 (1) Sequence Number: - Start / End Page: 373 - 379 Identifier: -