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  Thermodynamic and structural behaviour of α-Galactosylceramide and C6-functionalized α-GalCer in 2D layers at the air-liquid interface

Brezesinski, G., Calow, A. D. J., Pereira, C. L., & Seeberger, P. H. (2020). Thermodynamic and structural behaviour of α-Galactosylceramide and C6-functionalized α-GalCer in 2D layers at the air-liquid interface. ChemBioChem: A European Journal of Chemical Biology, 21(1-2), 241-247. doi:10.1002/cbic.201900491.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-C7AD-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-56CD-3
Genre: Journal Article

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 Creators:
Brezesinski, Gerald1, Author              
Calow, Adam D. J.2, Author              
Pereira, Claney Lebev2, Author              
Seeberger, Peter H.3, Author              
Affiliations:
1Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863286              
2Peter H. Seeberger - Vaccine Development, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863308              
3Peter H. Seeberger - Automated Systems, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863306              

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Free keywords: α-Galactosylceramide C6-functionalized α-GalCer monolayers thermodynamics structure
 Abstract: α-Galactosylceramide (KRN7000) is a ligand for the glycoprotein CD1d that presents lipid antigens to natural killer T cells. Therefore, KRN7000 as well as some modified version thereof have been widely investigated as part of novel immunotherapies. To examine the impact of structural modification, we investigated KRN7000 and C6-modified KRN7000 at the air-liquid interface using monolayer isotherms, BAM, IRRAS, GIXD and TRXF. The amino group has no influence on the highly ordered sub-gel structures found at lateral pressures relevant for biological membranes. Neither lateral compression nor the protonation state of the amino group has a measurable effect on the lattice structure, which is defined by strong and rigid intermolecular hydrogen-bonds. However, the first-order phase transition found for the C6-functionalized α-GalCer is connected with an extraordinary surface inhibited nucleation. Our study demonstrates that KRN7000 can be functionalized at C6 without significantly changing the structural properties.

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Language(s): eng - English
 Dates: 2019-09-222020
 Publication Status: Published in print
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 Identifiers: DOI: 10.1002/cbic.201900491
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Title: ChemBioChem: A European Journal of Chemical Biology
  Other : ChemBioChem
Source Genre: Journal
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Publ. Info: Weinheim, Germany : Wiley-VCH
Pages: - Volume / Issue: 21 (1-2) Sequence Number: - Start / End Page: 241 - 247 Identifier: ISSN: 1439-4227