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  Construction and applications of a B vitamin genetic resource for investigation of vitamin dependent metabolism in maize

Suzuki, M., Wu, S., Mimura, M., Alseekh, S., Fernie, A. R., Hanson, A. D., et al. (2020). Construction and applications of a B vitamin genetic resource for investigation of vitamin dependent metabolism in maize. The Plant Journal, 101(2), 442-454. doi:10.1111/tpj.14535.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-CA2D-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0005-81CA-5
Genre: Journal Article

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 Creators:
Suzuki, Masaharu1, Author
Wu, Shan1, Author
Mimura, Manaki1, Author
Alseekh, S.2, Author              
Fernie, A. R.2, Author              
Hanson, Andrew D.1, Author
McCarty, Donald R.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: Zea mays, B vitamins, biotin, pyridoxine, pipecolic acid, UniformMu, transposon mutagenesis
 Abstract: Abstract The B vitamins provide essential co-factors for central metabolism in all organisms. In plants, B vitamins have surprising emerging roles in development, stress tolerance and pathogen resistance. Hence, there is a paramount interest in understanding the regulation of vitamin biosynthesis as well as the consequences of vitamin deficiency in crop species. To facilitate genetic analysis of B vitamin biosynthesis and functions in maize, we have mined the UniformMu transposon resource to identify insertional mutations in vitamin pathway genes. A screen of 190 insertion lines for seed and seedling phenotypes identified mutations in biotin, pyridoxine and niacin biosynthetic pathways. Importantly, isolation of independent insertion alleles enabled genetic confirmation of genotype-to-phenotype associations. Because B vitamins are essential for survival, null mutations often have embryo lethal phenotypes that prevent elucidation of subtle, but physiologically important, metabolic consequences of sub-optimal (functional) vitamin status. To circumvent this barrier, we demonstrate a strategy for refined genetic manipulation of vitamin status based on construction of heterozygotes that combine strong and hypomorphic mutant alleles. Dosage analysis of pdx2 alleles in endosperm revealed that endosperm supplies pyridoxine to the developing embryo. Similarly, a hypomorphic bio1 allele enabled analysis of transcriptome and metabolome responses to incipient biotin deficiency in seedling leaves. We show that systemic pipecolic acid accumulation is an early metabolic response to sub-optimal biotin status highlighting an intriguing connection between biotin, lysine metabolism and systemic disease resistance signaling. Seed-stocks carrying insertions for vitamin pathway genes are available for free, public distribution via the Maize Genetics Cooperation Stock Center.

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Language(s): eng - English
 Dates: 20192020
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1111/tpj.14535
BibTex Citekey: doi:10.1111/tpj.14535
 Degree: -

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Title: The Plant Journal
  Other : Plant J.
Source Genre: Journal
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Publ. Info: Oxford : Blackwell Science
Pages: - Volume / Issue: 101 (2) Sequence Number: - Start / End Page: 442 - 454 Identifier: ISSN: 0960-7412
CoNE: https://pure.mpg.de/cone/journals/resource/954925579095_1