English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  The inducible response of the nematode Caenorhabditis elegans to members of its natural microbiota across development and adult life

Yang, W., Petersen, C., Pees, B., Zimmermann, J., Waschina, S., Dirksen, P., et al. (2019). The inducible response of the nematode Caenorhabditis elegans to members of its natural microbiota across development and adult life. Frontiers in Microbiology, 10: 1793. doi:10.3389/fmicb.2019.01793.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
fmicb-10-01793.pdf (Publisher version), 4MB
Name:
fmicb-10-01793.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show
hide
Locator:
Link (Publisher version)
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Yang, Wentao, Author
Petersen, Carola, Author
Pees, Barbara, Author
Zimmermann, Johannes, Author
Waschina, Silvio, Author
Dirksen, Philipp, Author
Rosenstiel, Philip, Author
Tholey, Andreas, Author
Leippe, Matthias, Author
Dierking, Katja, Author
Kaleta, Christoph, Author
Schulenburg, Hinrich1, Author           
Affiliations:
1Max Planck Fellow Group Antibiotic Resistance Evolution, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_2600692              

Content

show
hide
Free keywords: Caenorhabditis elegans, microbiota, Ochrobactrum, transcriptomics, RNA-Seq, metabolic network model
 Abstract: The biology of all organisms is influenced by the associated community of microorganisms. In spite of its importance, it is usually not well understood how exactly this microbiota affects host functions and what are the underlying molecular processes. To rectify this knowledge gap, we took advantage of the nematode Caenorhabditis elegans as a tractable, experimental model system and assessed the inducible transcriptome response after colonization with members of its native microbiota. For this study, we focused on two isolates of the genus Ochrobactrum. These bacteria are known to be abundant in the nematode’s microbiota and are capable of colonizing and persisting in the nematode gut, even under stressful conditions. The transcriptome response was assessed across development and three time points of adult life, using general and C. elegans-specific enrichment analyses to identify affected functions. Our assessment revealed an influence of the microbiota members on the nematode’s dietary response, development, fertility, immunity, and energy metabolism. This response is mainly regulated by a GATA transcription factor, most likely ELT-2, as indicated by the enrichment of (i) the GATA motif in the promoter regions of inducible genes and (ii) of ELT-2 targets among the differentially expressed genes. We compared our transcriptome results with a corresponding previously characterized proteome data set, highlighting a significant overlap in the differentially expressed genes, the affected functions, and ELT-2 target genes. Our analysis further identified a core set of 86 genes that consistently responded to the microbiota members across development and adult life, including several C-type lectin-like genes and genes known to be involved in energy metabolism or fertility. We additionally assessed the consequences of induced gene expression with the help of metabolic network model analysis, using a previously established metabolic network for C. elegans. This analysis complemented the enrichment analyses by revealing an influence of the Ochrobactrum isolates on C. elegans energy metabolism and furthermore metabolism of specific amino acids, fatty acids, and also folate biosynthesis. Our findings highlight the multifaceted impact of naturally colonizing microbiota isolates on C. elegans life history and thereby provide a framework for further analysis of microbiota-mediated host functions.

Details

show
hide
Language(s): eng - English
 Dates: 2019-01-212019-07-222019-08-072019
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.3389/fmicb.2019.01793
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Frontiers in Microbiology
  Abbreviation : Front. Microbiol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Lausanne : Frontiers Media
Pages: - Volume / Issue: 10 Sequence Number: 1793 Start / End Page: - Identifier: ISSN: 1664-302X
CoNE: https://pure.mpg.de/cone/journals/resource/1664-302X