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  Iron concentrations in neurons and glial cells with estimates on ferritin concentrations

Reinert, A., Morawski, M., Seeger, J., Arendt, T., & Reinert, T. (2019). Iron concentrations in neurons and glial cells with estimates on ferritin concentrations. BMC Neuroscience, 20: 25. doi:10.1186/s12868-019-0507-7.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-C78B-F Version Permalink: http://hdl.handle.net/21.11116/0000-0004-E53F-4
Genre: Journal Article

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 Creators:
Reinert, Anja, Author
Morawski, Markus, Author
Seeger, Johannes, Author
Arendt, Thomas, Author
Reinert, Tilo1, Author              
Affiliations:
1Department Neurophysics (Weiskopf), MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_2205649              

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Free keywords: Astrocytes; Elemental analysis; Ferritin; Iron; Microglia; Neurons; Oligodendrocytes; PIXE
 Abstract: BACKGROUND: Brain iron is an essential as well as a toxic redox active element. Physiological levels are not uniform among the different cell types. Besides the availability of quantitative methods, the knowledge about the brain iron lags behind. Thereby, disclosing the mechanisms of brain iron homeostasis helps to understand pathological iron-accumulations in diseased and aged brains. With our study we want to contribute closing the gap by providing quantitative data on the concentration and distribution of iron in neurons and glial cells in situ. Using a nuclear microprobe and scanning proton induced X-ray emission spectrometry we performed quantitative elemental imaging on rat brain sections to analyze the iron concentrations of neurons and glial cells. RESULTS: Neurons were analyzed in the neocortex, subiculum, substantia nigra and deep cerebellar nuclei revealing an iron level between [Formula: see text] and [Formula: see text]. The iron concentration of neocortical oligodendrocytes is fivefold higher, of microglia threefold higher and of astrocytes twofold higher compared to neurons. We also analyzed the distribution of subcellular iron concentrations in the cytoplasm, nucleus and nucleolus of neurons. The cytoplasm contains on average 73 of the total iron, the nucleolus-although a hot spot for iron-due to its small volume only 6 of total iron. Additionally, the iron level in subcellular fractions were measured revealing that the microsome fraction, which usually contains holo-ferritin, has the highest iron content. We also present an estimate of the cellular ferritin concentration calculating [Formula: see text] ferritin molecules per [Formula: see text] in rat neurons. CONCLUSION: Glial cells are the most iron-rich cells in the brain. Imbalances in iron homeostasis that lead to neurodegeneration may not only be originate from neurons but also from glial cells. It is feasible to estimate the ferritin concentration based on measured iron concentrations and a reasonable assumptions on iron load in the brain.

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Language(s): eng - English
 Dates: 2019-03-012019-05-212019-05-29
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1186/s12868-019-0507-7
BibTex Citekey: Reinert:2019
PMID: 31142282
PMC: PMC6542065
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Project name : Interdisziplinäre Ansätze in den zellulären Neurowissenschaften (InterNeuro) / GRK 1097
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Funding organization : German Research Foundation (DFG)
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Funding program : Young Scientist Award
Funding organization : German Federal Ministry for Education and Research (BMBF)
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Funding organization : The Saxon Ministry of the Arts and Sciences

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Title: BMC Neuroscience
Source Genre: Journal
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Publ. Info: BioMed Central
Pages: - Volume / Issue: 20 Sequence Number: 25 Start / End Page: - Identifier: ISSN: 1471-2202
CoNE: https://pure.mpg.de/cone/journals/resource/111000136905018