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  3D analysis of an induced atherosclerotic lesion in a murine artery by PIXE stacking

Sickert, A., Barapatre, N., Rothermel, M., Teupser, D., & Reinert, T. (2012). 3D analysis of an induced atherosclerotic lesion in a murine artery by PIXE stacking. International Journal of PIXE, 22: 01n02, pp. 81-86. doi:10.1142/S0129083512400244.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-CE03-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-CE04-0
Genre: Journal Article

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 Creators:
Sickert, Annemarie1, Author
Barapatre, Nirav1, Author
Rothermel, Martin1, Author
Teupser, Daniel1, Author
Reinert, Tilo1, Author              
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1External Organizations, ou_persistent22              

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Free keywords: 3D; stacking; atherosclerosis
 Abstract: Quantitative three dimensional analysis is possible, in principle, by PIXE tomography. But, the inherent problems in quantitation, restrictions on the sample geometry and preparation, and specimen damage due to high fluences make this method unsuitable for many biological samples. The specimen under investigation, a murine artery, was around a millimeter in diameter and the induced atherosclerotic lesion was spread few hundreds of micrometers across the length of the artery. Since no tomographic experiments were possible, we chose to do the 3D quantitative analysis by means of PIXE Stacking. Herein, thin serial sections of the specimen are prepared and measured by conventional ion beam techniques. The resultant two dimensional quantitative element maps are stacked and aligned to reconstruct a quantitative volume of the specimen. Although the reconstructed dimension has poorer spatial resolution as compared with the measured dimensions, new information can still be gained from it. The three dimensional element distribution of the atherosclerotic lesion shows calcification on the outer surface of the artery, which otherwise would not have been easily visible in the two dimensional analysis.

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Language(s): eng - English
 Dates: 2012-06-082011-11-302012-09-25
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1142/S0129083512400244
BibTex Citekey: Sickert:2012
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Title: International Journal of PIXE
Source Genre: Journal
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Pages: - Volume / Issue: 22 Sequence Number: 01n02 Start / End Page: 81 - 86 Identifier: -