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  Proton beam writing of microstructures in Agar gel for patterned cell growth

Larisch, W., Koal, T., Werner, R., Hohlweg, M., Reinert, T., & Butz, T. (2011). Proton beam writing of microstructures in Agar gel for patterned cell growth. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 269(20), 2444-2447. doi:10.1016/j.nimb.2011.02.041.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-CE0C-8 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-CE0D-7
Genre: Journal Article

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 Creators:
Larisch, Wolfgang1, Author
Koal, Torsten1, Author
Werner, Ronald1, Author
Hohlweg, Marcus1, Author
Reinert, Tilo1, Author              
Butz, Tilman1, Author
Affiliations:
1Nukleare Festkörperphysik, University of Leipzig, Germany, ou_persistent22              

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Free keywords: Proton beam writing; Agar gel; Microstructures; Cell growth
 Abstract: A rather useful prerequisite for many biological and biophysical studies, e.g., for cell–cell communication or neuronal networks, is confined cell growth on micro-structured surfaces. Solidified Agar layers have smooth surfaces which are electrically neutral and thus inhibit receptor binding and cell adhesion. For the first time, Agar microstructures have been manufactured using proton beam writing (PBW). In the irradiated Agar material the polysaccharides are split into oligosaccharides which can easily be washed off leaving Agar-free areas for cell adhesion. The beam diameter of 1 μm allows the fabrication of compartments accommodating single cells which are connected by micrometer-sized channels. Using the external beam the production process is very fast. Up to 50 Petri dishes can be produced per day which makes this technique very suitable for biological investigations which require large throughputs.

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Language(s): eng - English
 Dates: 2011-03-092011-10-15
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.nimb.2011.02.041
BibTex Citekey: Larisch:2011
 Degree: -

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Title: Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
  Abbreviation : NIM B
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Amsterdam : Elsevier B.V.
Pages: - Volume / Issue: 269 (20) Sequence Number: - Start / End Page: 2444 - 2447 Identifier: ISSN: 0168-583X
CoNE: https://pure.mpg.de/cone/journals/resource/954925484704