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  Scanning transmission ion microscopy tomography at the Leipzig nanoprobe LIPSION

Reinert, T., Sakellariou, A., Schwertner, M., Vogt, J., & Butz, T. (2002). Scanning transmission ion microscopy tomography at the Leipzig nanoprobe LIPSION. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 190(1-4), 266-270. doi:10.1016/S0168-583X(01)01186-7.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-C56D-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-C56E-3
Genre: Journal Article

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 Creators:
Reinert, Tilo1, Author              
Sakellariou, A.2, Author
Schwertner, M.1, Author
Vogt, J.1, Author
Butz, T.1, Author
Affiliations:
1Fakultät für Physik und Geowissenschaften, Nukleare Festkörperphysik, University of Leipzig, Germany, ou_persistent22              
2Former at the Microanalytical Research Center, School of Physics, The University of Melbourne, Australia, ou_persistent22              

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Free keywords: Ion microbeam; Scanning transmission ion microscopy; Tomography; Subcellular; Cartilage; Collagen
 Abstract: Scanning transmission ion microscopy (STIM) of joint cartilage could visualise single collagen fibrils. Thus, answers to the controversial questions of their alignment could be given. However, the fibrils form three-dimensional structures that are not yet fully disclosed. STIM tomography is needed to give more detailed information. The size of the structures requires a challenging resolution of about 100 nm. The first STIM tomographic experiment has been performed at the Leipzig nanoprobe LIPSION. 360 projections of a cartilage sample () were taken. The pixel resolution was 250×250 pixels for each projection. The data set was reconstructed at MARC Melbourne using the backprojection of filtered projections technique. The data show the feasibility of STIM tomography in cartilage research. However, experimental inaccuracies (rotational displacement and magnetic stray fields) have limited the resolution thus far. Improvements in the experimental set-up will lead to higher resolution.

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Language(s): eng - English
 Dates: 2002-01-062002-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1016/S0168-583X(01)01186-7
BibTex Citekey: Reinert:2002b
 Degree: -

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Title: Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
  Abbreviation : NIM B
Source Genre: Journal
 Creator(s):
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Publ. Info: Amsterdam : Elsevier B.V.
Pages: - Volume / Issue: 190 (1-4) Sequence Number: - Start / End Page: 266 - 270 Identifier: ISSN: 0168-583X
CoNE: https://pure.mpg.de/cone/journals/resource/954925484704