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  Mutations in NDUFS1 Cause Metabolic Reprogramming and Disruption of the Electron Transfer

Ni, Y., Hagras, M. A., Konstantopoulou, V., Mayr, J. A., Stuchebrukhov, A. A., & Meierhofer, D. (2019). Mutations in NDUFS1 Cause Metabolic Reprogramming and Disruption of the Electron Transfer. Cells, 8(10): E1149. doi:10.3390/cells8101149.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-C63F-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-C640-4
Genre: Journal Article

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 Creators:
Ni, Yang1, Author              
Hagras, Muhammad A. , Author
Konstantopoulou, Vassiliki , Author
Mayr, Johannes A. , Author
Stuchebrukhov , Alexei A. , Author
Meierhofer, David1, Author              
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1Mass Spectrometry (Head: David Meierhofer), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479669              

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Free keywords: complex I (CI) deficiency; electron tunneling (ET); metabolome and proteome profiling; reactive oxygen species (ROS); respirasome assembly
 Abstract: Complex I (CI) is the first enzyme of the mitochondrial respiratory chain and couples the electron transfer with proton pumping. Mutations in genes encoding CI subunits can frequently cause inborn metabolic errors. We applied proteome and metabolome profiling of patient-derived cells harboring pathogenic mutations in two distinct CI genes to elucidate underlying pathomechanisms on the molecular level. Our results indicated that the electron transfer within CI was interrupted in both patients by different mechanisms. We showed that the biallelic mutations in NDUFS1 led to a decreased stability of the entire N-module of CI and disrupted the electron transfer between two iron-sulfur clusters. Strikingly interesting and in contrast to the proteome, metabolome profiling illustrated that the pattern of dysregulated metabolites was almost identical in both patients, such as the inhibitory feedback on the TCA cycle and altered glutathione levels, indicative for reactive oxygen species (ROS) stress. Our findings deciphered pathological mechanisms of CI deficiency to better understand inborn metabolic errors.

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Language(s): eng - English
 Dates: 2019-09-202019-09-25
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.3390/cells8101149
PMID: 31557978
 Degree: -

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Title: Cells
Source Genre: Journal
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Publ. Info: Basel, Switzerland : MDPI AG (Multidisciplinary Digital Publishing Institute)
Pages: - Volume / Issue: 8 (10) Sequence Number: E1149 Start / End Page: - Identifier: ISSN: 2073-4409