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  The hexa- and pentapeptide extension of proalbumin II. Processing of specific antibodies against the synthetic hexapaptide

Weigand, K., Birr, C., & Suter, M. (1981). The hexa- and pentapeptide extension of proalbumin II. Processing of specific antibodies against the synthetic hexapaptide. Biochimica et Biophysica Acta: BBA, 670(3), 424-427. doi:10.1016/0005-2795(81)90116-1.

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BBA_670_1981_424.pdf (Any fulltext), 349KB
 
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 Creators:
Weigand, K., Author
Birr, Christian1, Author           
Suter, M., Author
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1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              

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Free keywords: Proalbumin; ELISA; Peptide synthesis; Albumin; Antibody development; Immunodiffusion
 Abstract: The chemically synthesized proalbumin hexapeptide was coupled to rabbit albumin with carbodiimide. Subsequently rabbits were immunized by subcutaneous injection of the conjugate. After 5 weeks the rabbits had developed antibodies against the hexapeptide, which could be detected by immunodiffusion. A sensitive enzyme-linked immunosorbent assay was developed. Using the hexapeptide and other very similar peptides as antigens, a high specificity of the antibodies against the proalbumin hexapeptide was found.

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Language(s): eng - English
 Dates: 1981-05-072003-02-051981-10-28
 Publication Status: Issued
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0005-2795(81)90116-1
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Title: Biochimica et Biophysica Acta : BBA
  Other : Biochimica et Biophysica Acta (BBA) - Biomembranes
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 670 (3) Sequence Number: - Start / End Page: 424 - 427 Identifier: Other: 1879-2642
CoNE: https://pure.mpg.de/cone/journals/resource/18792642