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  Pan-neuronal calcium imaging with cellular resolution in freely swimming zebrafish

Kim, D., Kim, J., Marques, J., Hildebrand, D., Gu, W., Li, J., et al. (2017). Pan-neuronal calcium imaging with cellular resolution in freely swimming zebrafish. Nature methods, 14(11), 1107-1114. doi:10.1038/nmeth.4429.

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https://www.nature.com/articles/nmeth.4429.pdf (Publisher version)
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Kim, DH, Author
Kim, J, Author
Marques, JC, Author
Hildebrand, DGC, Author
Gu, W, Author
Li, JM1, Author              
Robson, DN1, Author              
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1External Organizations, ou_persistent22              

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 Abstract: Calcium imaging with cellular resolution typically requires an animal to be tethered under a microscope, which substantially restricts the range of behaviors that can be studied. To expand the behavioral repertoire amenable to imaging, we have developed a tracking microscope that enables whole-brain calcium imaging with cellular resolution in freely swimming larval zebrafish. This microscope uses infrared imaging to track a target animal in a behavior arena. On the basis of the predicted trajectory of the animal, we applied optimal control theory to a motorized stage system to cancel brain motion in three dimensions. We combined this motion-cancellation system with differential illumination focal filtering, a variant of HiLo microscopy, which enabled us to image the brain of a freely swimming larval zebrafish for more than an hour. This work expands the repertoire of natural behaviors that can be studied with cellular-resolution calcium imaging to potentially include spatial navigation, social behavior, feeding and reward.

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 Dates: 2017-11
 Publication Status: Published in print
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 Identifiers: DOI: 10.1038/nmeth.4429
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Title: Nature methods
  Other : Nature methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 14 (11) Sequence Number: - Start / End Page: 1107 - 1114 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556