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  Photoactivation of silicon rhodamines via a light-induced protonation

Frei, M. S., Hoess, P., Lampe, M., Nijmeijer, B., Kueblbeck, M., Ellenberg, J., et al. (2019). Photoactivation of silicon rhodamines via a light-induced protonation. Nature Communications, 10: 4580, pp. 1-10. doi:10.1038/s41467-019-12480-3.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0004-D21D-F Version Permalink: https://hdl.handle.net/21.11116/0000-0004-D21E-E
Genre: Journal Article

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 Creators:
Frei, Michelle S.1, Author           
Hoess, Philipp, Author
Lampe, Marko, Author
Nijmeijer, Bianca, Author
Kueblbeck, Moritz, Author
Ellenberg, Jan, Author
Wadepohl, Hubert, Author
Ries, Jonas, Author
Pitsch, Stefan, Author
Reymond, Luc, Author
Johnsson, Kai1, Author           
Affiliations:
1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

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 Abstract: hotoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we create probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore’s outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.

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Language(s): eng - English
 Dates: 2019-05-092019-09-132019-10-08
 Publication Status: Published online
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41467-019-12480-3
 Degree: -

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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 10 Sequence Number: 4580 Start / End Page: 1 - 10 Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723