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  A low-cost laser-based nano-3D polymer printer for rapid surface patterning and chemical synthesis of peptide and glycan microarrays

Eickelmann, S., Tsouka, A., Heidepriem, J., Paris, G., Zhang, J., Molinari, V., et al. (2019). A low-cost laser-based nano-3D polymer printer for rapid surface patterning and chemical synthesis of peptide and glycan microarrays. Advanced Materials Technologies, 4(11): 1900503. doi:10.1002/admt.201900503.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-D827-D Version Permalink: http://hdl.handle.net/21.11116/0000-0005-DD2B-3
Genre: Journal Article

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 Creators:
Eickelmann, Stephan1, Author              
Tsouka, Alexandra1, Author              
Heidepriem, Jasmin1, Author              
Paris, Grigori1, Author              
Zhang, Junfang1, Author              
Molinari, Valerio2, Author              
Mende, Marco1, Author              
Löffler, Felix F.1, Author              
Affiliations:
1Felix Löffler, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2385692              
2Valerio Molinari, Kolloidchemie, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2385693              

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Free keywords: combinatorial synthesis, laser‐induced forward transfer, microarray, open source, solid phase synthesis
 Abstract: A low-cost laser-based printing setup is presented, which allows for the spot-wise patterning of surfaces with defined polymer nanolayers. These nanolayer spots serve as a “solid solvent,” embedding different chemicals, chemical building blocks, materials, or precursors and can be stacked on top of each other. By melting the spot pattern, the polymer-embedded molecules are released for chemical reaction. This enables researchers to quickly pattern a surface with different molecules and materials, mixing them directly on the surface for high-throughput chemical synthesis to generate and screen diverse microarray libraries. In contrast to expensive ink-jet or contact printing, this approach does not require premixing of inks, which enables in situ combinatorial mixing. Easy access and versatility of this patterning approach are shown by generating microarrays of various biomolecules, such as glycans for the first time, to screen interactions of antibodies and lectins. In addition, a layer-by-layer solid-phase synthesis of peptides directly on the microarray is presented. Amino acid–containing nanolayers are repeatedly laser-transferred and reacted with the functionalized acceptor surface in defined patterns. This simple system enables a reproducible array production, down to spot-to-spot distances of 100 μm, and offers a flexible and cheap alternative to expensive spotting robot technology.

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Language(s): eng - English
 Dates: 2019-10-092019
 Publication Status: Published in print
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 Identifiers: DOI: 10.1002/admt.201900503
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Title: Advanced Materials Technologies
Source Genre: Journal
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Publ. Info: Weinheim : Wiley
Pages: - Volume / Issue: 4 (11) Sequence Number: 1900503 Start / End Page: - Identifier: ISSN: 2365-709X