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  Stabilization of α-synuclein oligomers using formaldehyde

Ruesink, H., Reimer, L., Gregersen, E., Möller, A., Betzer, C., & Jensen, P. H. (2019). Stabilization of α-synuclein oligomers using formaldehyde. PLoS One, 14(10): e0216764. doi:10.1371/journal.pone.0216764.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-DA88-D Version Permalink: http://hdl.handle.net/21.11116/0000-0004-DA89-C
Genre: Journal Article

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 Creators:
Ruesink, Harm1, 2, Author
Reimer, Lasse1, 2, Author
Gregersen, Emil1, 2, Author
Möller, Arne3, Author              
Betzer, Cristine1, 2, Author
Jensen, Poul, Henning1, 2, Author
Affiliations:
1Danish Research Institute of Translational Neuroscience—DANDRITE, Aarhus University, Aarhus, Denmark, ou_persistent22              
2Department of Biomedicine, Aarhus University, Aarhus, Denmark, ou_persistent22              
3Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068291              

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 Abstract: The group of neurodegenerative diseases, Parkinson's disease (PD), dementia with Lewy bodies (DLB), and multiple system atrophy (MSA) all exhibit inclusions containing amyloid-type α-synuclein (α-syn) aggregates within degenerating brain cells. α-syn also exists as soluble oligomeric species that are hypothesized to represent intermediates between its native and aggregated states. These oligomers are present in brain extracts from patients suffering from synucleinopathies and hold great potential as biomarkers. Although easily prepared in vitro, oligomers are metastable and dissociate over time, thereby complicating α-syn oligomer research. Using the small amine-reactive cross-linker, formaldehyde (FA), we successfully stabilized α-syn oligomers without affecting their size, overall structure or antigenicity towards aggregate-conformation specific α-syn antibodies FILA and MJFR-14-6-4-2. Further, cross-linked α-syn oligomers show resistance towards denaturant like urea and SDS treatment and remain fully functional as internal standard in an aggregation-specific enzyme-linked immunosorbent assay (ELISA) despite prior incubation with urea. We propose that FA cross-linked α-syn oligomers could serve as important calibrators to facilitate comparative and standardized α-syn biomarker studies going forward.

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Language(s): eng - English
 Dates: 2019-04-232019-10-012019-10-11
 Publication Status: Published online
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1371/journal.pone.0216764
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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 14 (10) Sequence Number: e0216764 Start / End Page: - Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850