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  A human liver cell atlas reveals heterogeneity and epithelial progenitors

Aizarani, N., Saviano, A., Sagar, S., Mailly, L., Durand, S., Herman, J. S., et al. (2019). A human liver cell atlas reveals heterogeneity and epithelial progenitors. Nature, 572, 199-204. doi:10.1038/s41586-019-1373-2.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0004-E42B-B Version Permalink: http://hdl.handle.net/21.11116/0000-0004-E628-C
Genre: Journal Article

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 Creators:
Aizarani, Nadim1, Author
Saviano, Antonio2, Author
Sagar, Sagar1, Author
Mailly , Laurent2, Author
Durand, Sarah2, Author
Herman, Josip S.1, Author
Pessaux, Patrick2, Author
Baumert , Thomas F.2, Author
Grün, Dominic1, Author              
Affiliations:
1Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, 79108 Freiburg, DE, ou_2243640              
2External Organizations, ou_persistent22              

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 Abstract: The human liver is an essential multifunctional organ. The incidence of liver diseases is rising and there are limited treatment options. However, the cellular composition of the liver remains poorly understood. Here we performed single-cell RNA sequencing of about 10,000 cells from normal liver tissue from nine human donors to construct a human liver cell atlas. Our analysis identified previously unknown subtypes of endothelial cells, Kupffer cells, and hepatocytes, with transcriptome-wide zonation of some of these populations. We show that the EPCAM+ population is heterogeneous, comprising hepatocyte-biased and cholangiocyte populations as well as a TROP2int progenitor population with strong potential to form bipotent liver organoids. As a proof-of-principle, we used our atlas to unravel the phenotypic changes that occur in hepatocellular carcinoma cells and in human hepatocytes and liver endothelial cells engrafted into a mouse liver. Our human liver cell atlas provides a powerful resource to enable the discovery of previously unknown cell types in normal and diseased livers.

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Language(s): eng - English
 Dates: 2019-06
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41586-019-1373-2
 Degree: -

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Title: Nature
  Abbreviation : Nature
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 572 Sequence Number: - Start / End Page: 199 - 204 Identifier: ISSN: 0028-0836
CoNE: https://pure.mpg.de/cone/journals/resource/954925427238