English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Deciphering the Regulatory Landscape of ɣδ T Cell Development by Single-Cell RNA Sequencing

Sagar, S., Pokrovskil, M., Herman, J. S., Naik, S., Sock, E., Lausch, U., et al. (2018). Deciphering the Regulatory Landscape of ɣδ T Cell Development by Single-Cell RNA Sequencing. BioRxiv.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0004-E913-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-E914-F
Genre: Paper

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Sagar, Sagar1, Author
Pokrovskil, Maria2, Author
Herman, Josip S.2, Author
Naik, Shruti2, Author
Sock, Elisabeth2, Author
Lausch, Ute2, Author
Wegner, Michael2, Author
Tanriver, Yakup2, Author
Littman, Dan R.2, Author
Grün, Dominic1, Author              
Affiliations:
1Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243642              
2External Organizations, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: Recent studies have established γδ T cells as critical players in a broad range of infections, antitumor surveillance, autoimmune diseases and tissue homeostasis. However, differentiation of γδ T cells in the adult thymus remains poorly understood, due to the rare frequency of this lineage. Here, we infer high-resolution developmental trajectories of this rare population by single-cell RNA-sequencing. We reveal previously unknown subtypes and identify the transcription factor c-MAF as a novel key regulator of IL-17-producing γδ T cell (γδT17) differentiation. c-MAF knockout mice exhibit a complete block in γδT17 differentiation, absence of these cells from peripheral organs, and protection from an autoimmune phenotype in a psoriasis model. Single-cell RNA-sequencing of Sox13 and Rorc knockout mice pinpoints c-MAF as an essential missing link between these lineage-specifying factors. These findings significantly enhance our understanding of γδ T cell ontogeny. Our experimental strategy provides a blueprint for deciphering differentiation of rare cell types.

Details

show
hide
Language(s): eng - English
 Dates: 2018
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: No review
 Identifiers: DOI: org/10.1101/478529
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: BioRxiv
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: -