English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Immediate effects of ammonia shock on transcription and composition of a biogas reactor microbiome

Fischer, M. A., Ulbricht, A., Neulinger, S. C., Refai, S., Waßmann, K., Künzel, S., et al. (2019). Immediate effects of ammonia shock on transcription and composition of a biogas reactor microbiome. Frontiers in Microbiology, 10: 2064. doi:10.3389/fmicb.2019.02064.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0004-EB8C-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0004-EB8D-5
Genre: Journal Article

Files

show Files
hide Files
:
fmicb-10-02064.pdf (Publisher version), 2MB
Name:
fmicb-10-02064.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Fischer, Martin A., Author
Ulbricht, Andrea, Author
Neulinger, Sven C., Author
Refai, Sarah, Author
Waßmann, Kati, Author
Künzel, Sven1, Author              
Schmitz, Ruth A., Author
Affiliations:
1Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445635              

Content

show
hide
Free keywords: anaerobic digestion, biogas, ammonia, next-generation sequencing, 16S microbial community structure, metatranscriptomics
 Abstract: The biotechnological process of biogas production from organic material is carried out by a diverse microbial community under anaerobic conditions. However, the complex and sensitive microbial network present in anaerobic degradation of organic material can be disturbed by increased ammonia concentration introduced into the system by protein-rich substrates and imbalanced feeding. Here, we report on a simulated increase of ammonia concentration in a fed batch lab-scale biogas reactor experiment. Two treatment conditions were used simulating total ammonia nitrogen concentrations of 4.9 and 8.0 g/L with four replicate reactors. Each reactor was monitored concerning methane generation and microbial composition using 16S rRNA gene amplicon sequencing, while the transcriptional activity of the overall process was investigated by metatranscriptomic analysis. This allowed investigating the response of the microbial community in terms of species composition and transcriptional activity to a rapid upshift to high ammonia conditions. Clostridia and Methanomicrobiales dominated the microbial community throughout the entire experiment under both experimental conditions, while Methanosarcinales were only present in minor abundance. Transcription analysis demonstrated clostridial dominance with respect to genes encoding for enzymes of the hydrolysis step (cellulase, EC 3.2.1.4) as well as dominance of key genes for enzymes of the methanogenic pathway (methyl-CoM reductase, EC 2.8.4.1; heterodisulfide reductase, EC 1.8.98.1). Upon ammonia shock, the selected marker genes showed significant changes in transcriptional activity. Cellulose hydrolysis as well as methanogenesis were significantly reduced at high ammonia concentrations as indicated by reduced transcription levels of the corresponding genes. Based on these experiments we concluded that, apart from the methanogenic archaea, hydrolytic cellulose-degrading microorganisms are negatively affected by high ammonia concentrations. Further, Acholeplasma and Erysipelotrichia showed lower abundance under increased ammonia concentrations and thus might serve as indicator species for an earlier detection in order to counteract against ammonia crises. © Copyright © 2019 Fischer, Ulbricht, Neulinger, Refai, Waßmann, Künzel and Schmitz.

Details

show
hide
Language(s): eng - English
 Dates: 2019-04-112019-08-212019-09-062019
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.3389/fmicb.2019.02064
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Frontiers in Microbiology
  Abbreviation : Front. Microbiol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Lausanne : Frontiers Media
Pages: - Volume / Issue: 10 Sequence Number: 2064 Start / End Page: - Identifier: ISSN: 1664-302X
CoNE: https://pure.mpg.de/cone/journals/resource/1664-302X