English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Global characterization of the oocyte‐to‐embryo transition in Caenorhabditis elegans uncovers a novel mRNA clearance mechanism

Stoeckius, M., Grün, D., Kirchner, M., Ayoub, S., Torti, F., Piano, F., et al. (2014). Global characterization of the oocyte‐to‐embryo transition in Caenorhabditis elegans uncovers a novel mRNA clearance mechanism. EMBO Journal, 33, 1751-1766. doi:org/10.15252/embj.201488769.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Stoeckius, Marlon1, Author
Grün, Dominic2, Author           
Kirchner, Marieluise1, Author
Ayoub, Salah1, Author
Torti, Francesca1, Author
Piano, Fabio1, Author
Herzog, Margareta1, Author
Selbach, Matthias1, Author
Rajewsky, Nikolaus1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243642              

Content

show
hide
Free keywords: -
 Abstract: The oocyte‐to‐embryo transition (OET) is thought to be mainly driven by post‐transcriptional gene regulation. However, expression of both RNAs and proteins during the OET has not been comprehensively assayed. Furthermore, specific molecular mechanisms that regulate gene expression during OET are largely unknown. Here, we quantify and analyze transcriptome‐wide, expression of mRNAs and thousands of proteins in Caenorhabditis elegans oocytes, 1‐cell, and 2‐cell embryos. This represents a first comprehensive gene expression atlas during the OET in animals. We discovered a first wave of degradation in which thousands of mRNAs are cleared shortly after fertilization. Sequence analysis revealed a statistically highly significant presence of a polyC motif in the 3′ untranslated regions of most of these degraded mRNAs. Transgenic reporter assays demonstrated that this polyC motif is required and sufficient for mRNA degradation after fertilization. We show that orthologs of human polyC‐binding protein specifically bind this motif. Our data suggest a mechanism in which the polyC motif and binding partners direct degradation of maternal mRNAs. Our data also indicate that endogenous siRNAs but not miRNAs promote mRNA clearance during the OET.

Details

show
hide
Language(s): eng - English
 Dates: 2014
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: org/10.15252/embj.201488769
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: EMBO Journal
  Other : EMBO J.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 33 Sequence Number: - Start / End Page: 1751 - 1766 Identifier: ISSN: 0261-4189
CoNE: https://pure.mpg.de/cone/journals/resource/954925497061