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  Cloning of bacteriophage fd gene 2 and construction of a plasmid dependent on fd gene 2 protein

Meyer, T. F., & Geider, K. (1981). Cloning of bacteriophage fd gene 2 and construction of a plasmid dependent on fd gene 2 protein. Proceedings of the National Academy of Sciences of the United States of America, 78(9), 5416-5420. doi:10.1073/pnas.78.9.5416.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0004-F1AC-A Version Permalink: https://hdl.handle.net/21.11116/0000-0004-F1AD-9
Genre: Journal Article

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 Creators:
Meyer, Thomas F.1, Author           
Geider, Klaus2, Author           
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1Department of Molecular Biology, Max Planck Institute for Infection Biology, Max Planck Society, ou_1664147              
2Research Group Prof. Dr. Geider, Max Planck Institute for Medical Research, Max Planck Society, ou_1497738              

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 Abstract: Bacteriophage fd gene 2 was cloned in plasmid pBR325. Cells carrying the hybrid plasmid produce about 200 times more enzymatically active fd gene 2 protein than did cells infected with phage fd wild type, as measured by replication of phage fd replicative form I in vitro. Cloned gene 2 supports replication of an artificial phage fd miniplasmid consisting of the origin of bacteriophage fd replication and a gene coding for kanamycin resistance. This plasmid occurs in high copy numbers and is viable only in cells carrying the cloned fd gene 2 or in cells infected with phage fd. Because the miniplasmid is not propagated in natural hosts, it can be considered a safe cloning vector. Its fusion with the gene 2 hybrid plasmid provides an autonomous replicon independent of the polA function of the host cell. fd gene 2 is the only phage-encoded trans-acting function required for replication of double-stranded fd DNA in vivo.

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Language(s): eng - English
 Dates: 1981-05-281981-09-151981-09-01
 Publication Status: Issued
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1073/pnas.78.9.5416
URI: https://www.ncbi.nlm.nih.gov/pubmed/6272301
URI: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC348756/
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : Proc. Acad. Sci. USA
  Other : Proc. Acad. Sci. U.S.A.
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : PNAS
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 78 (9) Sequence Number: - Start / End Page: 5416 - 5420 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230