English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis

Hampoelz, B., Schwarz, A., Ronchi, P., Bragulat-Teixidor, H., Tischer, C., Gaspar, I., et al. (2019). Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis. Cell, 179(3), 671-686.e17. doi:10.1016/j.cell.2019.09.022.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0004-F644-A Version Permalink: http://hdl.handle.net/21.11116/0000-0005-3D67-4
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Hampoelz, Bernhard1, 2, Author              
Schwarz, Andre2, 3, Author
Ronchi, Paolo4, Author
Bragulat-Teixidor, Helena4, Author
Tischer, Christian5, Author
Gaspar, Imre6, Author
Ephrussi, Anne6, Author
Schwab, Yannick4, 7, Author
Beck, Martin1, 2, 7, Author              
Affiliations:
1Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society, ou_3040395              
2European Molecular Biology Laboratory, Structural and Computational Biology Unit, Heidelberg, Germany, ou_persistent22              
3Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of Biosciences, ou_persistent22              
4European Molecular Biology Laboratory, Electron Microscopy Core Facility, Heidelberg, Germany, ou_persistent22              
5Center for Bioimage Analysis, European Molecular Biology Laboratory, Heidelberg, Germany, ou_persistent22              
6European Molecular Biology Laboratory, Developmental Biology Unit, Heidelberg, Germany, ou_persistent22              
7European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Heidelberg, Germany, ou_persistent22              

Content

show
hide
Free keywords: local translation, microtubule-dependent transport, NPC assembly, nuclear pore complex, nucleoporin condensation, Nup358, oogenesis, Ran, RNA localization
 Abstract: The molecular events that direct nuclear pore complex (NPC) assembly toward nuclear envelopes have been conceptualized in two pathways that occur during mitosis or interphase, respectively. In gametes and embryonic cells, NPCs also occur within stacked cytoplasmic membrane sheets, termed annulate lamellae (AL), which serve as NPC storage for early development. The mechanism of NPC biogenesis at cytoplasmic membranes remains unknown. Here, we show that during Drosophila oogenesis, Nucleoporins condense into different precursor granules that interact and progress into NPCs. Nup358 is a key player that condenses into NPC assembly platforms while its mRNA localizes to their surface in a translation-dependent manner. In concert, Microtubule-dependent transport, the small GTPase Ran and nuclear transport receptors regulate NPC biogenesis in oocytes. We delineate a non-canonical NPC assembly mechanism that relies on Nucleoporin condensates and occurs away from the nucleus under conditions of cell cycle arrest.

Details

show
hide
Language(s): eng - English
 Dates: 2019-08-092018-11-202019-09-202019-102019-10-17
 Publication Status: Published in print
 Pages: 34
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.cell.2019.09.022
BibTex Citekey: hampoelz_nuclear_2019
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Cell
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 179 (3) Sequence Number: - Start / End Page: 671 - 686.e17 Identifier: ISSN: 0092-8674
CoNE: https://pure.mpg.de/cone/journals/resource/954925463183