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  Phosphoenolpyruvate-dependent phosphotransferase system of Staphylococcus aureus: 1H nuclear magnetic resonance studies on phosphorylated and unphosphorylated factor IIIlac and its interaction with the phosphocarrier protein HPr

Kalbitzer, H. R., Deutscher, J., Hengstenberg, W., & Rösch, P. (1981). Phosphoenolpyruvate-dependent phosphotransferase system of Staphylococcus aureus: 1H nuclear magnetic resonance studies on phosphorylated and unphosphorylated factor IIIlac and its interaction with the phosphocarrier protein HPr. Biochemistry, 20(21), 6178-6185. doi:10.1021/bi00524a041.

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Genre: Journal Article
Alternative Title : Phosphoenolpyruvate-dependent phosphotransferase system of Staphylococcus aureus: hydrogen-1 nuclear magnetic resonance studies on phosphorylated and unphosphorylated factor IIIlac and its interaction with the phosphocarrier protein HPr

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Kalbitzer, Hans Robert1, Author           
Deutscher, Josef2, Author           
Hengstenberg, Wolfgang2, Author           
Rösch, Paul1, Author           
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1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              
2Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              

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 Abstract: The trimeric phosphocarrier protein factor III specific for galactosides was investigated by 1H NMR spectroscopy. The protomer contains four histidyl residues with acidic pK values in the range 5.6-6.2. One of the histidyl residues, His-B, carries the phosphoryl group. The pK value of His-B increases from 6.0 to 8.6 upon phosphorylation. To determine the position of the phosphoryl group with respect to the nitrogens required the isolation of a peptide T-2 containing the phosphorylated active-center histidine and one of the other histidines. The pK value and the chemical shift of the phosphopeptide clearly indicated the phosphorus to be bound to the N-3 atom of the imidazole ring. The temperature dependence of the factor III spectrum demonstrates multiple conformations which exchange rapidly on the NMR time scale. Titration of factor III with HPr protein showed an upfield shift of the active-center histidine, indicating complex formation between both proteins. Phosphorylation of both proteins abolished the interaction, which is plausible from mechanistic considerations.

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Language(s): eng - English
 Dates: 1981-02-201981-10-13
 Publication Status: Published in print
 Pages: 8
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 Rev. Type: Peer
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Title: Biochemistry
Source Genre: Journal
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Publ. Info: Columbus, Ohio : American Chemical Society
Pages: - Volume / Issue: 20 (21) Sequence Number: - Start / End Page: 6178 - 6185 Identifier: ISSN: 0006-2960
CoNE: https://pure.mpg.de/cone/journals/resource/954925384103